| Project/Area Number |
04670796
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Iwate Medical University School of Medicine |
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Principal Investigator |
ISHIDA Kaoru Iwate Med.Univ.Schol.of Med.Instructor, 医学部, 講師 (70146041)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Keni-ichrou Iwate Med.Univ.Schol.of Med.Assistnt, 医学部, 助手 (20254758)
SATO Nobuhiro Iwate Med.Univ.Schol.of Med.Assistant, 医学部, 助手 (10244914)
TERASHIMA Masanori Iwate Med.Univ.Schol.of Med.Instructor, 医学部, 講師 (40197794)
TAMURA Gen Iwate Med.Univ.Schol.of Med.Assistant, 医学部, 助手 (20207244)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Tumor suppressor gene / p53 / cell sorting / Rb / MCC / APC / DCC / Microsatellite / 食道癌 / Microstatellite / PCR / 変異 |
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| Research Abstract |
To investigate the prognostic significance of the p53 gene abnormalities, mutation and LOH of the gene were examined by the cell sorting combined single-strand conformation polymorphism (PCR-SSCP) analysis in esophageal squamous cell carcinoma (ESCC). Further, four genetic alterations of the tumor suppressor genes (Rb, MCC,APC and DCC) were also examined by the PCR-LOH (loss of heterozygosity) assay or PCR-SSCP analysis. The cell sorting combined with PCR-SSCP analysis should improved the sensitivity of detecting p53 mutations, and provides additional information concerning the DNA ploidy pattern in endoscopic specimens of ESCCs. Although aberrations of the p53 gene were well correlated with patients' prognoses, remaining other tumor suppressor genes were not. LOH at the 3p25 detected by the microsatellite assay more freguently occurred in carcinomas with lymph node metastasis than in without it. Thus, the combination of genetic alterations examined may provide good informations in clinical use.
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