| Project/Area Number |
04670813
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Kurume University School of Medicine |
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Principal Investigator |
MORODOMI Tatsuhisa KURUME UNI.SCH.of MED.1st Dep.Assistant, 医学部, 助手 (80166462)
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| Co-Investigator(Kenkyū-buntansha) |
MIURA Kazuhide KURUME UNI.SCH.of MED.1st Dep.Assistant, 医学部, 助手 (20209711)
OHKITA Akira KURUME UNI.SCH.of MED.1st Dep.Assistant, 医学部, 助手 (50203724)
AKAGI Yoshito KURUME UNI.SCH.of MED.1st Dep.Assistant, 医学部, 助手 (90192880)
SHIROUZU Kazuo KURUME UNI.SCH.of MED.1st Dep.Lecturer, 医学部, 講師 (20216203)
緒方 裕 久留米大学, 医学部, 助手 (20177124)
笹栗 靖之 産業医科大学, 教授 (60140646)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | metabloproteinase / invasion / metastasis / metastatic model / MMp-9 / Timp-1 / nude mouse / nude rat / In site Hybridization / ヌードラット / ヒト大腸癌 / 肝転移 / セリンプロテアーゼ |
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| Research Abstract |
Natural metastatic model is necessary to make clear the metastatic mechanism of cancer cells. We made the liver metastatic model from caecal tumor in nude mouse and the lung metastatic model from caccal tumor in nude rat using culture cells of human colon cancer. We studied about the expression of metalloproteinases (MMP-2,9, TIMP-1), adhesion molecules (SLe^N, SLe^n, CD44) and CEA in both metastatic models.
1.MMP-2 and 9 showed the strong secretion from caecal tumor in zymogram. Especially, MMP-9 produced by tumor cell was strongly correlated with liver metastasis.
2.In the adhesion melecules, the caecal tumor with liver metastasis in nude mouse showed the high expression of SLe^x and that with lung metastasis in nude rat showed the high expression of CD44.
3.The CEA value was very high in blood of caecal tumor with liver and lung metastasis. We suggested that the CEA value correlated with the volume of tumor cells departed in blood vessel.
4.Both metastatic models have many interesting characteristics. These models are useful to make clear mechanism of cancer metastasis and the experiment of adjuvant chemotherapy and immumotherapy.
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