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Immunohistochemical Study of Glutathione-related Enzyme and Proliferative Antigen in Non-Small Cell Lung Cancer

Research Project

Project/Area Number 04670841
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionTOKAI UNIVERSITY

Principal Investigator

OGAWA Jun-ichi  Tokai University, Associate Professor, 医学部, 助教授 (20112774)

Co-Investigator(Kenkyū-buntansha) IWASAKI Masayuki  Tokai University, School of Medicine, 医学部, 助手 (90223388)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
KeywordsNon-small cell lung cancer / Glutathione peroxidase / Glutathione reductase / Proliferating cell nuclear antigen / Epidermal growth factor receptor / Cisplatin / グルタチオン / 増殖抗原 / CDDP感受性 / グルタチン関連酵素
Research Abstract

With resected tumor tissue from 107 patients with non-small cell lung cancer, the expression of glutathione peroxidase (GPX), glutathione reductase (GR), proliferating cell nuclear antigen (PCNA), and epidermal growth factor receptor (EGFR) was examined in relation to CDDP sensitivity. The CDDP sensitivity was assessed from an increase of cells in the S-phase or G2M-phase by a DNA histogram after the tumor cells were incubated in a CDDP solution. The expression of GPX,GR,PCNA and EGFR for each tumor was studied with an indirect immunoperoxidase technique on paraffin-embedded tissues.
The percentage of patients sensitive to CDDP according to the histologic type was 40% for large cell carcinomas, 31% for squamous cell carcinomas, and 6% for adenocarcinomas. There was an inverse relationship between CDDP sensitivity and the frequency of GPX and GR expression. The GPX and GR expression was significantly lower in the CDDP-sensitive group than in the CDDP-resistant group. However, the expression of PCNA and EGFR was significantly lower in the sensitive group in non-small cell lung cancer.
From the above findings, an immunohistochemical study of these antigens may be useful for predicting CDDP sensitivity in non-small cell lung cancer.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 小川純一: "肺癌におけるグルタチオン関連酵素と増殖抗原の免疫組織研究" CANCER. 71. 2204-2209 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 山田俊介: "非小細胞肺癌における腫瘍増殖能とCDDP感受性の関連" 肺癌. 32. 367-374 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Jun-ichi Ogawa: "Immunohistochemical Study of Glutathione Related Enzymes and Proliferative Antigens in Lung Cancer-Relation to Cisplatin Sensitivity-" CANCER. 71. 2204-2209 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shunsuke Yamada: "The Relationship between Tumor Proliferative Potential and CDDP Sensitivity in Non-small Cell Lung Cancer." Lung Cancer. 32. 367-374 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小川純一: "肺癌におけるグルタチオン関連酵素と増殖抗原の免疫組織研究" CANCER. 71. 2204-2209 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 山田俊介: "非小細胞肺癌における腫瘍増殖能とCDDP感受性の関連" 肺癌. 32. 367-374 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] Jun-ichi Ogawa: "Immunohistuchemical study of glutathicne related enzymes and probferative antigens in lungcanar" Cancer. 71. 2204-2209 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Jun-ici Ogawa: "Immunohistochemical Study of Glutathione Related Enzymes and Proliferative Antigens in Lung Cancer:Relation to Cisplatin Sensitivity" Cancer. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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