| Project/Area Number |
04670844
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
FUJIWARA Takashi Kawasaki Medical School, Thoracic Surgery, Professor, 医学部, 教授 (90090224)
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Masami Kawasaki College of Allied Health Professions, Medical Electronics, Associate Pr, 医用電子技術科, 助教授 (50148699)
OGASAWARA Yasuo Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (10152365)
TSUJIOKA Katsuhiko Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (30163801)
KAJIYA Fumihiko Kawasaki Medical School, Medical Engineering, Professor, 医学部, 教授 (70029114)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | CCD microscope / Coronary circulation / Thoracic surgery / Microcirculation / Reactive hypermia / Subendocardial microvessel / Nitroglycerin |
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| Research Abstract |
To evaluate the microcirculation of the human myocardium morphologically and functionally, we developed a portable needle-probe video-microscope with a charge coupled device (CCD) camera was applicable to the coronary microvessels during a cardiac surgery. First, using open-chest anesthetized pigs and dogs, we aimed to find technological hurdles for introducing the system during a surgery. Then, we developed several pieces of equipment to overcome the methodological problems. In brief, a sheathed needle-probe with a doughnut-shaped balloon was developed to avoid direct compression of the microvessels by the tip of the needle. To maintain a physiological condition of myocardial microvessels and the surrounding tissue during observation, a system consisting of a heat exchanger and a microtube was developed for flushing away the intervening blood between the tip of the needle and the myocardium. Using these devices, images of the myocardial microvessels of a beating heart could be obtained for a time which was long enough for imaging study. Furthermore, a holding device of the portable needle-probe video-microscope with a CCD camera was developed, which allowed an operator easy handling. This holding device contributed to shorten observation time. For the myocardial microvessels to be visualized without any special intravascular contrast medium, we employed a green (complimentary color of red) filter to accentuate the contrast between the images of the blood filled vessel and the surrounding tissue. Then, not only the coronary arterioles, but also coronary venules could be visualized. We confirmed that the portable needle-probe video-microscope with a CCD camera was able to visualize human coronary microvessels using all these supporting systems.
We also evaluated the effects of nitroglycerin microvessels. The study showed that nitroglycerin dilates both subepicardial subendocardial arterioles. It was also found that nitroglycerin dilated coronary venules.
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