| Project/Area Number |
04670870
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Wakayama Medical College |
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Principal Investigator |
ITAKURA Toru Wakayama Medical College, Neurological Surgery, Professor, 医学部, 教授 (40100995)
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| Co-Investigator(Kenkyū-buntansha) |
FUNAHASHI Kazuyoshi Wakayama Medical College, Neurological Surgery, Assistant Professor, 医学部, 講師 (20142391)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | パーキンソン病 / 組織培養 / 神経成長因子 / Interleukin / 神経移植 / 交感神経節 / サイトカイン / 交感神経 |
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| Research Abstract |
By the support of Grant-in-Aid for Scientific Research (C) from 1992-1993, we have studied viability of transplanted sympathetic ganglion which had been cultured for a long period. In 1992, viability of the long-term cultured sympathetic ganglion was examined in 6-OHDA lesioned rats. The ganglion cultured for 2, or 4 weeks well survived in the host brain and revealed recovery of abnormal behavior of the 6-OHDA lesioned rats. In contrast ganglion cultured for 6 weeks failed to show survival of the graft and recovery of the behavior. In 1993, during culture of the ganglion, we added Interleukin-1 to induce nerve growth factor in the cultured medium. The sympathetic ganglion cultured in the Interleukin-1-conditioned medium showed high concentration of nerve growth factor(seven times higher than the control) and extended neurite outgrowth. Transplantation of the cultured ganglion in the Interleukin-1-conditioned medium revealed higher viability than the control in the host brain.
These results indicate the possibility of that the sympathetic ganglion can be treated in the culture system for 4 weeks to obtain high viability of the transplant, and this procedure can be clinically applied in the future.
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