| Project/Area Number |
04670874
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Juntendo University |
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Principal Investigator |
ARAI Hajime Juntendo Univ. Medical Department Associate Professor, 医学部, 助教授 (70167229)
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| Co-Investigator(Kenkyū-buntansha) |
KDOTA Yoshiaki Juntendo Univ. Medical Department Assistant, 医学部, 助手 (60204517)
UDO Akira Juntendo Univ. Medical Department Assistant, 医学部, 助手 (10185051)
YASUMOTO Yukimasa Juntendo Univ. Medical Department Assistant, 医学部, 助手 (20175649)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Hippocampus / Neuron / Glutamate / Calcium / Cell death / Hypothermia / 神経毒性 / 神経保護 / 培養神経細胞 / グリア / 神経保護作用 / 脳虚血 |
|---|
| Research Abstract |
To elucidate the precise mechanism of neuronal protection by brain hypothermia, we conducted an investigation as to what effect low temperature had on glutamate-induced intracellular free calcium ([Ca^<2+>]i) accumulation and cell death in cultured hippocampal neurons. Fifteen minutes of exposure to either 100muM or 1mM glutamate uniformly induced a marked increase in [Ca^<2+>]i, with delayd recovery and massive neuronal death under the conditions of both 37ﾟC and 30ﾟC.The study indicated that brain hypothermia cannot save neurons once glutamate is released during ischemia, and that intraischemic hypothermia in vivo probably prevents the development of ischemic neuronal injuly by suppressing extracellular glutamate release.
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