| Project/Area Number |
04670875
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Jikei University of Medicine |
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Principal Investigator |
NAKAHARA Shigehiro Jikei University, Neurosurgery, Lecturer, 医学部, 講師 (80147339)
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| Co-Investigator(Kenkyū-buntansha) |
SHUKUYA Ikuo Jikei University, Neurosurgery, assistant, 医学部, 助手 (90226291)
TANAKA Hideaki Jikei University, Neursurgery, assistant, 医学部, 助手 (70227164)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Chiari II malformation / Delayd Splotch mouse / Hydrocephalus / Myeloschisis / Spina bifida / hydrocephalus / myeloschisis / spina bifida / Chari II malformation / Luschka / Magendie / subarachnoid space / delayed Splotch |
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| Research Abstract |
We have already reported our unified theory of the cause of Chiari II malformation : failure of correct timing of apposition and transient occulusion of spinal neurocele, which leads to failure to maintain distention of the rhombencephalic vesicles, which alters the inductive effect of pressure on the surrounding mesenchyme. Therefore, we hypothesize the cause of hydrocephalus associated with myeloschisis is related to lack of driving force on the surrounding mesenchyme, especially arachnoid membrane. To test the possibility, we tried to investigate sagittal and coronal sections of the embryonic brain in normal and neural tube defect (NTD) in delayd Splotch mouse using Richardson's staining and the fetal arachnoid membrane of normal and NTD (myeloschisis) in delayd Splotch mouse using scanning electron microscope (SEM) and to make a baby model with myeloschisis using surgical glue for repairing myeloschisis to investigate arrachnoid membrane.
Finally, we failed to make a baby model with myeloschisis which caused by respiratory disorder associated with Chiari II malformation. However, SEM finding of fetal arachnoid membrane and subarachnoid space of the posterior fossa and vertex of the parenchyma in myeloschisis was quite different with that of normal fetus.
Our data suggest that the cause of hydrocephalus with Chiari II malformation associated with myeloschisis is related to this maldevelopment of the embryonic subarachnoid system, which leads to lack of distention of the embryonic ventricular system.
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