• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Detection of chromosome abnomality by fluorescent in situ hybridization in brain tumors.

Research Project

Project/Area Number 04670877
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionKANSAI MEDICAL UNIVERSITY

Principal Investigator

KAWAMOTO Keiji  KANSAI MEDICAL UNIVERSITY NEUROSURGERY PROFESSOR, 医学部, 教授 (70077741)

Co-Investigator(Kenkyū-buntansha) SAKAI Nobuyuki  KANSAI MEDICAL UNIVERSITY NEUROSURGERY ASSISTANT, 医学部, 助手 (30225768)
NUMA Yoshihiro  KANSAI MEDICAL UNIVERSITY NEUROSURGERY ASSISTANT, 医学部, 助手 (40208278)
KAWAKAMI Katsuhiro  KANSAI MEDICAL UNIVERSITY NEUROSURGERY ASSISTANT PROFESSOR, 医学部, 講師 (40186060)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsFISH / Brain Tumor / flow cytometry / chromosome / DNA-histogram / paraffin / Chromosome / brain tumor
Research Abstract

(1)Flow cytometric analysis of the cell cycle in brain tumors.
Flow cytometory (FCM) has been an effective method to abtain the biological aspects of brain tumors. We can clarify the mechanism of anticancer agents such as 1-hexycarbamoy 1-5 fluouracil, CDDP, MCNU and TNF.
(2)Expression of oncogene products in brain tumors.
In established brain tumor cells strains, immunohistochemical and flow cytometric methods are useful for evaluation of the positivity of c-myc products expression.
(3)Development of FISH method for detection of chromosome abnomality.
1)Chromosome numbers of 7,9,10,17 were dectected in glioma cell lines by FISH method.
2)Chromosome numbers of 7,17 were high in gliomas obtained by surgical operation.
3)Meningioma showed low chromosome numbers of 22, but high chromosome numbers of 22 in recurrent cases.
(4)Detection of chromosome abnomality with use of paraffin embeded specimen.
We succeeded to detect the chromosome numbers with use of paraffin blocks, which was correlated with fresh specimen.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 笠井治文、今堀巧、河本圭司: "FISH法によるglioma染色体異常の検出" HUMAN CELL. 6. 62-65 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 今堀巧、河本圭司: "flow cytometryによるPCNAをマーカーとした増殖期細胞の定量と細胞周期" HUMAN CELL. 6. 66-70 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] "The mecheauism of Action of 1-hexylcarbamoy1-5-flrorouracil by DNA/BrdU Double Staining Using Flow Cytometry." Progress in Oncology. 2(1). 55-61 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] "ANALYSIS OF DNA PLOIDY PAT-TERN IN BRAIN TUMORS" Neuroimmunological Research. 5. 178-183 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] "Detection of chromosomal numerical aberration in glioma by FISH(fluorescence in situ hybridization)" HUMAN CELL. 6(1). 62-65 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 今堀 巧,河本 圭司: "PCNAの基礎的検討-FCMによる検出と細胞同期との関連" Oncology の進歩. 2. 58-64 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakai Kawamoto: "Flow cytometry による脳腫瘍におけるc-myc遺伝子産物と核DNA量の同時定量的解析" cytometry Research. 3. (1993)

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi