| Project/Area Number |
04670879
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
KODAYASHI Shu Tokyo Metropolitan Institute of Gerontology Department of Neurophysiology, Investigator, 生理老化学研究系神経生理部門, 研究員 (30161988)
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| Co-Investigator(Kenkyū-buntansha) |
KAJI Kazuhiko Tokyo Metroolitan Institute of Gerontology Department of Biochemistry and Isotop, 老化科学技術研究系アイソトープ部門, 主査研究員 (40073019)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | glioma / growth factor / endothelial cell / cell culture |
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| Research Abstract |
We have been studying the growth-regulating mechanism of human glioma cells in vitro using several established cell lines. During investigtating serum-free culture methods, we fund SK-MG-1 cells derived from a human malignant astrocytoma grow without addition of any growth factors nor hormones. They inceased in number in MCDB-104 basal culture medium with almost the same speed as they do in serum-contained medium. We have been subcultivating SK-MG-1 cells serially more than 200 times in MCDB-104.
The conditioned medium of SK-MG-1 cells in the plateau phase but not the cell lysate effectively increased the numbers of human umbrical vein endothelial cells. This activity was weak in the conditioned medium of logarithmically growing SK-MG-1 cells. This glioma-derived endothelial cell growth factor (G-ECGF) activity did not bind the heparin sepharose nor show cross-activity with specific antibodies against basic FGF or acidic FGF.We purified the factor and the identification of the aminoacid sequence is in progress. G-ECGF had 17 kDa subunits with aparent molecular weight of around 30-40 kDa.
The conditioned medium of SK-MG-1 cells contained other factors, a new type of PDGF (AA, large form), a large amount of TGFbeta, and a factor which inhibits the growth of the parent cells. These factors including G-ECGF seemed to control the growth and survival of glioma cels and have influence on neighborhood cells such as endothelial cells. They may cntribute to explain the paracrine and autocrine regulation of human glioma cells in vivo.
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