| Project/Area Number |
04670887
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
整形外科学
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| Research Institution | MIE UNIVERSITY |
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Principal Investigator |
OGIHARA Yoshio MIE UNIVERSITY,FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (20024755)
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| Co-Investigator(Kenkyū-buntansha) |
SEKIGUCHI Shoji MIE UNIVERSITY,UNIVERSITY HOSPITAL,ASSISTANT, 医学部・付属病院, 助手 (50242946)
HUJINAMI Syuichi MIE UNIVERSITY,UNIVERSITY HOSPITAL,ASSISTANT, 医学部・付属病院, 助手 (30199351)
SHIOKAWA Yasuo MIE UNIVERSITY,FACULTY OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (80115708)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Chemosensitiritytest / Malignant tumor / Soft tissue sarema / Bloneytometry / Anitimor / Poliamin / Oncogene / Chemosensitirity test / malignant fare tumor / soft tissue sarcoma / flowcytomotry / mtitnior drug / oncogene / colony assay / chemotherapy / anti-tumor agent / flow cyto metry / sensitivity test / cell calture / fibroblast / flow cytometry / cell culture |
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| Research Abstract |
Chemosensitivity test is very useful method to detect the efficacy of chemotherapy for the patient with malignant bone and soft tissue tumor. In addition to the basical study of harvesting of tumor cells, study about histological findings and ploidy pattern with flowcytometry was performed. We reported correlation between the results and prognosis at the meeting of the Japan Orthopaedic Association. We analyzed antitumor effects of methotrexate, adriamycin, ifosfamide and methylglyoxal bis (cyclopentylamidinohydrazone) with osteosarcoma cell lines. The results that there were additional effects of general antitumor drug and metgylhlyoxal bis (cyclopentylamidinohydrazone) with different pharmacokinetics were reported. Now we have the results about analysis of effect to cell cycle by the method of using 5-Bromodeoxyuridine (BrdU), fluorescein isothiocyanate labeled anti-BrdU monoclonal antibody and propidium iodide with flowcytometry. It will be reported qt the meeting of the Japan Orthopaedic Association.
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