EXPERIMENTAL STUDY OF PERIPHERAL NERVE ALLOGRAFT WITH ETHANOL PRETREATMENT
| Project/Area Number |
04670901
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | KYOTO PREFECTURALUNIVERSITY OF MEDICINE |
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Principal Investigator |
TAMAI Kazuo DEPARTMENT OF ORTHOPAEDIC SURGERY,KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,, 医学部, 講師 (80227256)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | PERIPHERAL NERVE / ALLOGRAFT / ETHANOL / BASEMENTMEMBRANE / LAMININ |
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| Research Abstract |
TO REDUCE THE IMMUNOLOGICAL REACTIONS AGAINST THE ALLOGRAFTED NERVE IS THE MAJOR PROBLEM ABOUT PERIPHERAL NERVE ALLOGRAFT.TWO METHODS ARE SUJESTED TO REDUCE IMMNOREACTIONS.ONE IS TO USE IMMUNOSUPPRESSIVEAGENT AND THE OTHER IS TO REDUCE THE ANTIGENISITY OF THE GRAFTED NERVE BY PRETREATMENT.OUR COLLEAGUE HIRASAWA PLANED TO REDUCE THE ANTIGENISITY BY ETHANOL PRETREATMENT AND REPORTED GOOD RENERVATION.BUT THE RESULT WAS INFERIOR TO THAT OF THE AUTOGRAFT.IN THIS STUDY,WE IMPROVED THE WAY OF THE PRETREATMENT.FIRST,GRAFTED NERVE WAS PRETREATED BY 50,70,90 100% ETHANOL GRADUALLY EVERY FOUR HOURS.STIFFNESS OF THE BASEMENTMEMBRANE WAS OBSERVED AND THE LOW IMMUNOREACTION OF THE ANTI-LAMININE ANTIGEN WAS DETECTED IN THE IMMUNOCYTOCHEMISTRY.AT THREE MONTHS AFTER GRAFTING,THE RENERVATION THROUGH THE GRAFTED NERVE WAS NOT SO GOOD.THEN THE GRAFTED NERVE WAS PRETREATED BY 50,70,90% ETHANOL EVERY FOUR HOURS TWO TIMES.STIFFNESS OF THE BASEMENTMEMBRANE WAS IMPROVED AND THE IMMUNOREACTION OF THE LAMININ WAS AS SAME AS THAT OF THE NORMAL NERVE.GOOD RENERVATION WAS OBSERVED HITOLOGICALLY AND ELECTROPHYSIOLOGICALLY.BUT THE MATURATION OF THE REGENERATED AXON AND THE AMPLITUDE OF THE CMAP WAS INFERIOR TO THE RESULTS OF THE AUTOGRAFTED NERVE,AND FURTHER EXAMINATION WILL BE NEEDED FROM NOW ON.
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Report
(4 results)
Research Products
(11 results)
