| Project/Area Number |
04670911
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | OSAKA MEDICAL COLLEGE |
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Principal Investigator |
MORISHITA Shinobu OSAKA MEDICAL COLLEGE, Assistant Prof., 医学部, 講師 (90182229)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIZU Tunehiko OSAKA MEDICAL COLLEGE, Instructor, 医学部, 助手 (70222990)
ABE Muneaki OSAKA MEDICAL COLLEGE, Assistant Prof., 医学部, 助教授 (70084994)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Limb Lengthening / Experimental study / Histlogy / Muscle / Elongation |
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| Research Abstract |
Morphological changes of continuously stretched muscle in limblengthening were studied by histochemical methods using 14 growing rabbits. The tibialis anterior muscles were observed after 20% callus distraction of the tibia. The localization of regenerative and hypertrophic muscle fibers was studied by Acridine Orange staining in four portions (MTj., distal, central and proximal portion) of the muscle. The diameter of type 1 and Type 2 fibers in the central portion was measured by image analyzer. Our results showed that the primary site of muscle elongation was musclotendinous junction where regenerative muscle fibers were numerous. In the existing muscle fibers, Type 1 fibers showed hypertrophic change due to passive hyperstretching and Type 2 fibers showed disuse atrophy. But atrophic Type 2 fibers recovered gradually after the end of limb lengthening procedure.
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