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The basic reseach in clinical field with alpha-blocker and beta-stimulant therapy for male infertility

Research Project

Project/Area Number 04670959
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionNagoya University

Principal Investigator

MIYAKE Koji  Nagoya University Department of Urology ; Professor, 医学部, 教授 (70023752)

Co-Investigator(Kenkyū-buntansha) HIBI Hatsuki  Nagoya University Department of Urology ; Assistant instructor, 医学部, 助手 (70242865)
YAMAMOTO Masanori  Nagoya University Department of Urology ; Assistant instructor, 医学部, 助手 (90191444)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Keywordsepididymis / sperm parameter / a-blocker / α-遮断剤 / 精子濃度 / 精巣 / 微小穿刺
Research Abstract

This study was undertaken to investigate whether alpha blocker has an effect on epididymal sperm parameters, intraluminal pressure and fluid movement in the rat cauda epididymal tubules. Sprague Dawley rats were given daily oral doses 1mg/kg, 2mg/kg, or 10mg/kg of Bunazosin, which was a selective alpha blocker, for one month. Intraluminal fluids were collected from the cauda epididymidis using a micropuncture technique. Epididymal sperm concentrations were significantly increased after administration of alpha blocker. Progressive motility of apermatozoa was not significantly altered by alpha bloker. Intraluminal pressures were significantly reduced and intraluminal fluid movement rates were significantly increased by alpha blocker. Outside diameters of seminiferous and epididymal tubules were significantly distended when treated with alpha-blocker.
The increase in epididymal sperm concentration after alpha blocker administration seems to be associated with dilatation of the epididymal tubules that results in subsequente maintenance of reduced luminal pressure coupled with fast tubular fluid flow and increased sperm storage capacity. This research is a first step for additional investigations of the role of alpha-blocker in testicular or epididymal physiology and may pave way for human clinical application of alpha-blocker therapy in the treatment of male infertility.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Yamamoto M,Tsuji Y and Miyake K: "Microassay of sperm concentration in the rat epididymis by micropuncture technique." Nagoya J Med Sci. 55. 71-75 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y and Miyake K: "Transepithelial movement of ^3H-androgen in rat seminiferous and caput epididymal tubules:saturability and effect of estradiol." Nagoya J Med Sci. 56. 81-87 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y and Miyake K: "Evidence for active transport of ^3H-androgen across the epididymal epithelium in the rat." Nagoya J Med Sci. 56. 111-121 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Specificity of transepithelial movement of ^3H-androgen in rat seminiferous and caput epididymal tubules examined by in vivo microperifusion and in vivo micropuncture." Jpn J Fertil Steril. 39. 1-5 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake.K: "Epididymal sperm concentration determined by micropuncture technique and daily testicular sperm production in the rat." Jpn J Fertil Steril. 39. 229-232 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Hibi H and Miyake k: "Intraluminal concentrations of sodium,potassium,and sperm in the rat epididymis examined by in vivo micropuncture." Jpn J Fertil Steril. 39. 363-369 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Intraluminal content is required for the maintenance of antigrade proluminal movement of ^3H-androgens into rat caput epididymal tubules." Nagoya J Med Sci. 57. 77-83 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Effects of protein synthesis inhibitor and antimicrotubular agent on transepithelial movement of ^3H-androgens in the rat caput epididymis." Nagoya J Med Sci. 57. 119-126 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Effect of albumin on proluminal movement of ^3H-androgen into seminiferous and epididymal tubules and androgen binding in the interstitium of the testis and epididymis after perifusion with fluid containing albumin." Nagoya J Med Sci. 57. 133-142 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 山本雅憲、日比初紀,三宅弘浩: "男性不妊症治療の最近の進歩" 臨床泌尿器科. 48. 457-465 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y and Miyake K: "Microassay of sperm concentration in the rat epididymis by micropuncture technique." Nagoya J Med Sci. 55. 71-75 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y and Miyake K: "Transepithelial movement of ^3H-androgen in rat seminiferous and caput epididymal tubules : saturability and effect of estradiol." Nagoya J Med Sci. 56. 81-87 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y and Miyake K: "Evidence for avtive transport of ^3H-androgen across the epididymal epithelium in the rat." Nagoya J Med Sci. 56. 111-121 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Specificity of transepithelial movement of ^3H-androgen in rat seminiferous and caput epididymal tubules examined by in vivo microperifusion and in vivo micropuncture." Jpn J Fertil Steril. 39. 1-5 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Epididymal sperm concentration determined by micropuncture technique and daily testicular sperm production in the rat." Jpn J Fertil Steril. 39. 229-232 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Hibi H and Miyake K: "Intraluminal concentrations of sodium, potassium, and sperm in the rat epididymis examined by in vivo micropuncture." Jpn J Fertil Steril. 39. 363-369 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Intraluminal content is required for the maintenance of antigrade proluminal movement of ^3H-androgens into rat caput epididymal tubules." Nagoya J Med Sci. 57. 77-83 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Effects of protein synthesis inhibitor and antimicrotubular agent on transepithelial movement of ^3H-androgens in the rat caput epididymis." Nagoya J Med Sci. 57. 119-126 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Effect of albumin on proluminal movement of ^3H-androgen into seminiferous and epididymal tubules and androgen binding in the interstitium of the testis and epididymis after perifusion with fluid containing albumin." Nagoya J Med Sci. 57. 133-142 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto M,Tsuji Y and Miyake K: "Microassay of sperm concentration in the rat epididymis by micropuncture technique." Nagoya J Med Sci. 55. 71-75 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto M,Tsuji Y and Miyake K: "Transepithelial movement of ^3H-androgen in rat seminiferous and caput epididymal tubules: saturability and effect of estradiol." Nagoya J Med Sci. 56. 81-87 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto M,Tsuji Y and Miyake K: "Evidence for active transport of ^3H-androgen across the epididymal epithelium in the rat." Nagoya J Med Sci. 56. 111-121 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Specificity of transepithelial movement of ^3H-androgen in rat seminiferous and caput epididymal tubules examined by in vivo microperifusion and in vivo micropuncture." Jpn J Fertil Steril. 39. 1-5 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Epididymal sperm concentration determined by micropuncture technique and daily testicular sperm production in the rat." Jpn J Fertil Steril. 39. 229-232 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto M,Hibi H and Miyake K: "Intraluminal concentrations of sodium,potassium,and sperm in the rat epididymis examined by in vivo micropuncture." Jpn J Fertil Steril. 39. 363-369 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Intraluminal content is required for the maintenance of antigrade proluminal movement of ^3H-androgens into rat caput epididymal tubules." Nagoya J Med Sci. 57. 77-83 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Effects of protein synthesis inhibitor and antimicrotubular agent on transepithelial movement of ^3H-androgens in the rat caput epididymis." Nagoya J Med Sci. 57. 119-126 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto M,Tsuji Y,Hibi H and Miyake K: "Effect of albumin on proluminal movement of ^3H-androgen into seminiferous and epididymal tubules and androgen binding in the interstitium of the testis and epididymis after perifusion with fluid containing albumin." Nagoya J Med Sci. 57. 133-142 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 山本雅憲、日比初紀、三宅弘治: "男性不妊症治療の最近の進歩" 臨床泌尿器科. 48. 457-465 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto M.et.al.: "Microassay of sperm concentration in the rat epididymis by micropuncture technique." Nagoya J.Med.Sci.55. 71-75 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto M.et.al.: "Transepithelial movement of ^3H-androgen in rat seminiferous and caput epididymal tubules:saturability and effect of estradiol." Nagoya J.Med.Sci. 56. 81-87 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto M.et.al.: "Evidence for active transport of ^3H-androgens across the epididymal epithelium in the rat." Nagoya J.Med.Sci.56. 111-121 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto M.et.al.: "Specificity of transepithelial movement of ^3H-androgen in rat seminiferous and caput epididymal tubules examined by in vivo microperifusion and in vivo micropuncture." Jap.J.Fertil.Steril.39. 1-5 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto M.et.al.: "Intraluminal content is required for the maintenance of antigrade proluminal movement of ^3H-androgens into rat caput epididymal tubules." Nagoya J.Med.Sci.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto M.et.al.: "Effects of protein synthesis inhibitor and antimicrotubular agent on transepithelial movement of ^3H-androgens in the rat caput epididymis." Nagoya J.Med.Sci.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] YAMAMOTO,M.et al.: "The effect of hypophysectomy on proluminal movement of 3H-androgens across the epididymal epithelium in the rat." Nagoya:J.Med.Sci.54. 67-76 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] YAMAMOTO,M.et al.: "Microassay of Sperm Concentration in the rat epididymis by micropumcture technique." Nagoya.J.Med.Sci.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] YAMAMOTO,M.et al.: "Transepithelial muvement of 3H-androgen in rat seminiferous and caput epididymal tubules:Saturability and effect of estradiol" Nagoya.J.Med.Sci.(1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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