| Project/Area Number |
04670970
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | OITA MEDICAL UNIVERSITY |
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Principal Investigator |
MIZOGUCHI Hiroaki OITA MEDICAL UNIVERSITY, DEPARTMENT OF UROLOGY, ASSISTANT PROFESSOR, 医学部・泌尿器科, 講師 (40157524)
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| Co-Investigator(Kenkyū-buntansha) |
IMAGAWA Masaharu OITA MEDICAL UNIVERSITY, DEPARTMENT OF UROLOGY, ASSISTANT PROFESSOR, 医学部・泌尿器科, 助手 (10168510)
NAKAGAWA Masayuki OITA MEDICAL UNIVERSITY, DEPARTMENT OF UROLOGY, ASSISTANT PROFESSOR, 医学部・泌尿器科, 講師 (90164144)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | renal cell carcinoma / drug resistance / mdr1 / GST-pi / DNA topoisomerase II / トポイソメラーザII / 制癌剤耐性 |
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| Research Abstract |
We measured expression level of multidrug-resistant genes such as mdr1, glutathione S transferase gene (GST-pi), and DNA topoisomerase II(topoII) in human renal cell carcinoma tissue and normal renal tissue to study the mechanisms of drug-resistance in renal cell carcinoma. We also examined the effect of interferon alpha on the expression of these drug-resistance related genes. As a result, we observed similar level of mdr1 expression in renal cell carcinoma to that in normal renal tissue and lower level of GST pi expression in renal cell carcinoma than that in normal renal tissue. We also found that topo II gene expression was higher in renal cell carcinoma than normal tissue. Interferon treatment did not affect mdr1 expression but enhanced GST-pi expression and decreased topoII expression. These data suggest that mdr1 expression appears to be associated with drug-resistance in renal cell carcinoma, but it was still remained unclear that there is any correlation between gene expression of GST-pi and topo II and drug-resistant mechanism in renal cell carcinoma. Further study is necessary to elucidate these correlation in renal cell carcinoma.
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