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Bulk collection of epididymal tail spermatozoa by means of electric stimulation

Research Project

Project/Area Number 04670973
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionYokohama City University

Principal Investigator

KINOSHITA Yuzo  Yokohama City University, School of Medicine, Associate Professor, 医学部, 助教授 (00186298)

Co-Investigator(Kenkyū-buntansha) HOSAKA Masahiko  Yokohama City University, School of Medicine, Professor, 医学部, 教授 (30106330)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsEpididymis / Electric stimulation / Spermatozoa / 精子運動 / 電位負荷 / 副睾丸
Research Abstract

In 12-week-old Wistar rats, 70V,250 mu s pulse width, 20 Hz electric stimulation on the epididymal tail for 30 s resulted in a recovery of 10.5(]SY.+-。[)2.2 mu l of epididymal fluid containing 46 ((]SY.+-。[)14) x 10<@D16@>D1 spermatozoa. Simple dispersion of the collected epididymal fluid into F12 yielded pure suspensions of motile sperms. In the electric stimulation, spermatozoa may underwent loss of motility in two different mechanisms. In the condition of low ionic strength, 50% of motile spermatozoa lose motility in the high electric gradient about 160V/2mm. In the high ionic strength, spermatozoa lose motility even in low electric gradient but this takes places only near electrode. In men with obstructive diseases of the vas deferens, the epididymal spermatozoa could be collected by means of electric stimulation but not so consistently as in the animal experiments. The highest successful rate was observed in the cases where the obstruction is of the functional origin such as post-lymphadenectomy of the paraortic nodes.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 川上寧 他: "電気刺激によるラット精巣上体尾部精子の大量回収法" 日本不妊学会誌. 36. 872-875 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawakami et al: "Bulk collection of epididymal tail spermatozoa by means of electric stimulation in rats" Jpn J Fertil Steril. 36. 872-875 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 川上 寧: "電気刺激によるラット精巣上体尾部精子の大量回収法" 日本不妊学会誌. 36. 872-875 (1991)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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