| Project/Area Number |
04670989
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
MATSUI Hideo Chiba University School of Medicine, Assistant Professor, 医学部, 助手 (70190395)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OSADA Hisao Chiba University School of Medicine, Assistant Professor, 医学部, 助手 (30233505)
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | Choriocarcinoma / Hydatidiform mole / Tumor suppressing gene / DNA polymorphism / PCR |
|---|
| Research Abstract |
1. The pregnancies responsible for 6 choriocarcinoma cases were successfully indenrified by DNA polymarphism analysis using PCR method. Choriocarcinoma DNA samples were extracted from formalin-fixed, paraffin-embedded tissue(2 cases), tumors implanted on nude mice(2 cases), fresh poerational specimen(1 case). Four out of 6 choriocarcinonmas were determinded to derive from hydatidiform moles. Ouranalysis revealed an interesting case which was not derived from the last normal pregnancy, but a preceeding hydatidform mole. One out of these 4 moles has been already shown to exhibit chromosomal heterogenity. Addidionally, our analysis was cofirmed to ve useful for the differntial diagnosis of twin pregnancies with a mormal pregnancy and hydatidiform mole. One out of4 cases caused a choriocarcinoma, in which the hydatidiform mole showed chrmosomal heterogenity. 2. TheDNA polymorphisims, were demonstrated to ve remarkably informative and easily detected. Therfore, we amplified DNAsequences showing this type of polymorphism by PCR rechnique and anallysed differences in band pattern betweeneach sample. In paticular, microsatellites derived form fmre than 3 base-pair repeats were most useful for our analysis in terms of 1) simple detection system consisting ofPCR, electrophoresis on polyacrylamide gel, and silver staining and 2) wide and multiple distribution on each chromosome. 3. Whithin a given study period we failed to collect enough numbers of choriocarcinoma samples for satistical analysis and to determine the chromosomes showing constantly homozygosity in herero-moles. This wa arised form the rapid decrease in morbidity of the disease inself and the cases treated with operation. It is shus snceassary collect samples in collabration with other organizations.
|
