| Project/Area Number |
04671004
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
NAGAI Nobutaka Hiroshima Univ.School of Medicine, Assistant Professor, 医学部, 講師 (90198292)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Takahiro Hiroshima Univ.Medical Hospital Resident, 医学部・付属病院, 医員
OTA Sanae Hiroshima Univ.Medical Hospital Research Associate, 医学部・付属病院, 助手 (10243552)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Cervical Cancer / Human Papillomavirus / Oncogene / Tumor Suppressor Gene / Molecular Biology / Immuno Response / ヒトパピローマウイルス / 子宮頸癌 |
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| Research Abstract |
Molecular biological studies have shown that human papilloma-virus (HPV), some oncogenes and tumor suppressor genes are associated with uterine cervical carcinogenesis.We examined HPV DNA typing and its gene expression, oncogenes (c-myc, EGF-R) and p53 in cervical dysplasia and cancer with molecular biologic, immunohistochemical technique and binding assay.
The HPV study revealed that HPV type 16 and 18 DNA as well as EGF-R was detected at a high frequency at a higher grade of dysplasia and in the early stage of cervical cancer.In the oncogene study, c-myc gene overexpression was recongnized in the advanced stage of cervical cancer.The other oncogene and p53 were found in a low frequency and were non-specific genes in cervical cancer.In analysis of local immune, the number of S-100 positive cells and CD45RO cells increased as the grade of dysplasia was higher.
These findings indicated that HPV type 16/18 promote DNA synthesis throughout EGF-R and playd a role of initiation in cervical carcinogenesis and that some oncogenes and tumor suppressor genes were associated with cervical cancer.
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