| Project/Area Number |
04671007
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SANO Masatoshi Kyushu University Gynecol & Obstet Assist.professor, 医学部, 講師 (60206000)
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| Co-Investigator(Kenkyū-buntansha) |
NOZAKI Masahiro Kyushu University Gynecol & Obstet Assist.professor, 医学部, 助手 (60228319)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | ENDOMETRIOSIS / PERITONEAL MACROPHAGE / PHOSPHOLIPASE A2 / PROSTAGLANDINS / INTERLEUKIN-6 / TNF / 腹腔細胞 / マクロファージ |
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| Research Abstract |
It has recently been indicated that prostaglandin (PG) biosynthesis increases in the peritoneal fluid of women with endometriosis. In order to assess the PG production on peritoneal fluid cells (PFC), phospholipase A_2 activity of those cells in women with endometriosis was measured and compared with that of women without endometriosis. There were at least four measurable kinds of phospholipase A_2 activity detected in the cells, such as two calcium-dependent pH optima 7.0 and 9.0 activities as well as two calcium-independent pH optima 7.5 and 8.5 activities. One of those activities, the calcium-dependent and alkaline(pH 9.0) active enzyme, was significantly higher in women with endometriosis (n=15) as cmpared with that in women without endometriosis(n=9, p<0.01) both on a protein basis and cell number basis. These results indicate that the elevated prostaglandin levels in peritoneal fluid with endometriosis may be produced by PFC in which the phospholipase A_2 activity is elevated.
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