The cellular immuno-chemistrical study of intraperitoneal environment in patients with endometriosis.

• Project/Area Number: 04671007
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: KYUSHU UNIVERSITY
• Principal Investigator: SANO Masatoshi Kyushu University Gynecol & Obstet Assist.professor, 医学部, 講師 (60206000)
• Co-Investigator(Kenkyū-buntansha): NOZAKI Masahiro Kyushu University Gynecol & Obstet Assist.professor, 医学部, 助手 (60228319)
• Project Period (FY): 1992 – 1993
• Project Status: Completed (Fiscal Year 1993)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: ENDOMETRIOSIS / PERITONEAL MACROPHAGE / PHOSPHOLIPASE A2 / PROSTAGLANDINS / INTERLEUKIN-6 / TNF / 腹腔細胞 / マクロファージ

Research Abstract

It has recently been indicated that prostaglandin (PG) biosynthesis increases in the peritoneal fluid of women with endometriosis. In order to assess the PG production on peritoneal fluid cells (PFC), phospholipase A_2 activity of those cells in women with endometriosis was measured and compared with that of women without endometriosis. There were at least four measurable kinds of phospholipase A_2 activity detected in the cells, such as two calcium-dependent pH optima 7.0 and 9.0 activities as well as two calcium-independent pH optima 7.5 and 8.5 activities. One of those activities, the calcium-dependent and alkaline(pH 9.0) active enzyme, was significantly higher in women with endometriosis (n=15) as cmpared with that in women without endometriosis(n=9, p<0.01) both on a protein basis and cell number basis. These results indicate that the elevated prostaglandin levels in peritoneal fluid with endometriosis may be produced by PFC in which the phospholipase A_2 activity is elevated.

Research Products

• [Publications] Masatoshi Sano,et al: "Elevation of the phosphdipase A_2 activity in peritoneal fluid cells from women with endometriosis." Fertility and Sterility. 61. (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Masatoshi Sano,et al: "Changes of peritoneal fluid prostaglandins and peritoneal fluid cell's phospholipase A_2 activity in endometriasis women treated with Danzol." Fertility and Sterility. (投稿準備中).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Masatoshi Sano, Tetsuya Morishita, masahiro Nozaki, Motofumi Yokoyama, Yoshitsugu Watanabe, Hitoo Nakano.: "Elevation of the phospholipase A2 activity in peritoneal fluid cells from women with endometriosis." Fertility & Sterility. 61(4), (in press). (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Masatoshi Sano, Kazunori Hashimoto, Yoshihito Inoue, Masahiro Nozaki, Yoshitsugu Watanabe, Hitoo Nakano.: "Changes of peritoneal fluid prostaglandins and peritoneal fluid cell's phospholipase A2 activity in endometriosis women treated with Danazol." Fertility & sterility. (in preparation).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Masatoshi Sano,et al: "Elevation of the phospholipace A_2 activity in peritoneel fluid cells from women with endometriosis." Fertility and Sterility. 61. (1994)