| Project/Area Number |
04671014
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yokohama City University |
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Principal Investigator |
GORAI Itsuo (1992, 1994) Yokohama City University, Sch.of Med., Dept.Obst.& Gyn., 医学部, 講師 (70162170)
五來 逸雄 (1993) 横浜市立大学, 医学, 講師
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| Co-Investigator(Kenkyū-buntansha) |
NAKAZAWA Tsuneo Yokohama City University, Sch.of Med., Dept.Obst.& Gyn., 医学部, 助手 (90254169)
HIRAHARA Fumiki Yokohama City University, Sch.of Med., Dept.Obst.& Gyn., 医学部, 講師 (30201734)
平吹 知雄 横浜市立大学, 医学部, 助手 (80244440)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | endometriosis / antiendometrial antibody / ELISA / serum / peritoneal fluid / GnPH agonist / GnRll agonist |
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| Research Abstract |
Immunologic phenomena have been postulated in the etiology of endometriosis. The association of antiendometrial tissue-specific antibodies with endometriosis has been proposed, suggesting an abnormal polyclonal B-cell activation-a classic characteristic of autoimmune disease. Our aim was to investigate endometrial antigens involved in the autoimmunity of endometriosis. We first applied the ELISA method to detect antiendometrial antibodies in the serum and peritoneal fluid of endometriosis patients as well as normal females and found that antiendometrial antibody levels were sighificantly higher in the serum of endometriosis patients than those in normal controls without endometriosis (absorbance at 405nm : 0.414<plus-minus> 0.120 vs 0.249 <plus-minus>0.103). We could not find any differences in absorbance among groups with different r-AFS stages. The absorbance of 11 endometriosis patients decreased after treatment with GnRH agonist (0.791 to 0.656). Then we detected endometrial antigens against which autoantibodies directed with Western blotting. Thirteen (72.2%), 14 (77.8%), and 15 (83.3%) of 18 serum samples from endometriosis patients had antibodies reactive against endometrial antigen with MW of 26kd, 34kd, and 42kd, respectively, while 6 (33.3%), 8 (44.4%), and 8 (44.4%) of 18 samples from normal control women reacted against these antigens, respectively. The frequencies of antibodies to the endometrial antigens were significantly (p<0.05) higher in the endometriosis patients than in the normal control woman. Isolation and characterization of endometrial antigens might help further understanding of the pathogenesis of endometriosis as well diagnosis of endometriosis patients without laparoscopic intervent
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