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The research for energistic metabolism of cancer using the transplantable cancer model of rabbit VX2-virus derived cancer cells.

Research Project

Project/Area Number 04671024
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 産婦人科学
Research InstitutionTokyo Women's Medical College

Principal Investigator

TAKIZAWA Ken  TWMC,Faculty of Medicine Associate Professor, 医学部, 助教授 (10107683)

Co-Investigator(Kenkyū-buntansha) IKUTA Masaaki  TWMC,Faculty of Medicine Instructor, 医学部, 助手
KAKINOKI Shigeko  TWMC,Faculty of Medicine Instructor, 医学部, 助手 (70224350)
MATUSHIRO Naomi  TWMC,Faculty of Medicine Instructor, 医学部, 助手 (40219431)
HARADA Makoto  TWMC,Faculty of Medicine Instructor, 医学部, 助手 (60208684)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
KeywordsMagnetic resonance spectroscopy (MRS) / Ploliferative cell nuclear antigen (PCNA) / P53 / Flowcytometry / DNA ploidy pattern / Urinary plasminogen activator receptor (uPAR) / Plasminogen activator inhibitor-1 (PAI-1) / Malignant potential / Proliferating cell nuclear antigen(PCNA) / 変異型癌抑制遺伝子産物(P53) / uPA / 免疫組織化学 / HPLC法 / 高エネルギー燐酸化合物 / 子宮肉腫 / PCNA / P53 / 免疫組織染色 / エネルギー代謝 / Magnetic Resonance Spectroscopy / VX2ウイルス由来癌細胞 / ヌードマウス移植癌 / ヒト卵巣腺癌 / HPLC
Research Abstract

The abilities of tumor growth and metastasis reflecting the malignant potential were evaluated.
(1) Magnetic resonance spectroscopy (MRS) was studied for the rabbit cancer model 2 weeks after transplantation with VX2- virus derived cells (1*10^6 cells/0.1ml). As the tumor size increased, the figure of Pcr/Pi on the MRS wave decreased. Pcr/Pi values was changed from 5.4 to 1.2 immediately after the intravenous injection of ADM (15mg), but recovered to 7.6 at 30 minutes later. Following the local injection of ADM (1.5mg), Pcr/Pi gradually decreased from 3.7*2.6*1.0.
(2) Biochemical determination of energistic metabolites by HPLC method for human ovarian tumor revealed more energistic metabolites in benign tissue than those in cancer.
Both physiological and biochemical determinations of energistic metabolites might not reflect the clinical malignant potential.
(3) Immunohistochemical staining of PCNA and P53 revealed strong positivity in 4 among 8 patients with leiomyosarcoma. In combination with immunohistochemistry, DNA ploidy pattern were determined by flowcytometry ; 1.3<plus-minus>0.1 of DNA index, 2.3<plus-minus>0.2 of number of peak channel and 39<plus-minus>4 of the ratio of diploidy in leiomyosarcoma, which were significantly different from benign g
(4) The coagulative and fibrinolytic factors were determined by EIA as follows ; UPA 47<plus-minus>33, uPAR 62<plus-minus>34 and PAI-1 21<plus-minus>11 ng/ml in ovarian cancer, which were higher values than those of benign tissue. As for the metastatic focus, uPAR levels were 2-3 times higher, and PAI-1,10-20 times higher than those of the primary site. Furthermore, PAI-1 was significantly immunostained in the interstitial tissue of the metastatic
The results suggest that uPAR and PAI-1 might be promoting factors of the metastasis.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] 滝沢憲他10名: "AngiotensinII併用Cisplatin, Sodium thiosulfate二経路化学療法の薬物動態的検討" 日本産婦人科学会誌. 44. 595-602 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 滝沢憲他4名: "妊娠に合併した子宮頚部高度異形成・上皮肉癌および浸潤癌の細胞診" 日本臨床細胞学会誌. 32. 900-905 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 滝沢憲: "女性性器癌に対する抗癌剤の選択と卵巣機能に及ぼす影響" Oncology and Chemotherapy. 9. 11-15 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 滝沢憲: "卵巣癌の治療-最近の動向-保存手術" KARKINOS. 7. 1001-1004 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 原田誠,滝沢憲他4名: "進行卵巣癌に対するNeoadjuvant Chemotherapyの効果" Oncology and Chemotherapy. 9. 250-258 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 原田誠: "卵巣癌後腹膜リンパ節転移の予後に与える意義とリンパ節転移に対する抗癌剤投与法の実験的検討" 日本癌治療学会誌. 29. 1725-1734 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 滝沢憲: "がん化学療法の副作用対策" 脱毛とその対策 先端医学社(東京), 325-335pp (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 滝沢憲: "図説産婦人科VIEW-12,腫瘍:薬物療法" 副作用対策-性機能障害 メジカルビュー(東京), 194-200pp (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takizawa, K.et al: "The clinical and pharmacokinetic evaluation of two-route chemotherapy with cisplatine and sodium thiosulfate in combination with angiotensin II." Acta.Obst.Gynaec.Jpn.44. 595-602 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takizawa, K.et al: "The clinical evaluation of cytological diagnosis during pregnancy in the management of severe dysplasia, carcinoma in situ and invastive cancer complicating pregnancy." J.Jpn.Soc.Clin.Cytol.32. 900-905 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Harada, M., Takizawa, K.et al: "The effect of neoadjuvant chemotherapy for the extremely advanced cancer of the ovary." Oncology and Chemotherapy. 9. 250-258 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takizawa, K.: "The application of the chemotherapeutic agents aginst cancers of the female genital tract and their toxicity for the ovarian function" Oncology and Chemotherapy. 9. 11-15 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Harada, M.: "The clinical effect of retroperitoneal lymphnode metastasis on therapeutic results in ovarian cancer patients and the experimental evaluation of anticancer effect by the different administrative routes to lymphnode metastasis." J.Jpn.Soc.Cancer Ther.29. 1735-1734 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takizawa, K.: "The recent trend in the management for ovarian cancer-Function retaining surgery for ovarian cancer." Karkinos. 7. 1006-1009 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takizawa, K.et al: "The clinical evaluation of serum CEA and SCC values in patients with cancer of the uterine cervix." Oncology and Chemotherapy. 8. 373-379 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takizawa, K.: "Intraperitoneal infusion of CDDP combined with angiotensisn II and sodium thiosulfate with special reference to its beneficial effects of decreasing toxicity and increasing anticancer function." Oncology and Chemotherapy. 9. 308-312 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takizawa, K.: "Anticancer agents and their harmful effects on the ovarian function." Obstet.Gynaecol.61. 355-361 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 滝沢憲: "尿路感染症の診断と治療。産婦人科から泌尿器科へ望むこと。" 化学療法の領域. 10. 862-866 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 滝沢憲: "婦人科手術におけるMRSA感染症の予防対策。" 産科と婦人科. 61. 355-361 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 滝沢憲: "抗癌剤と卵巣機能" 産科と婦人科. 61. 355-361 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 滝沢憲: "Human Papilloma Virusと発癌" 臨床婦人科産科. 48. 427-428 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 滝沢憲 他3名: "薬物性血液障害-汎血液障害" 医薬ジャーナル. 30. 1617-1622 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 滝沢憲: "卵巣癌の治療-最近の動向-保存手術" KARKINOS. 7. 1001-1009 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 滝沢憲: "図説産婦人科VIEW-3,手術:性器脱・形成手術" 後膣壁形成術。メジカルビュー(東京), 50-67 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 滝沢憲: "図説産婦人科VIEW-12,腫瘍:薬物療法" 副作用対策-性機能障害。メジカルビュー(東京), 194-200 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 滝沢憲: "産婦人科臨床におけるクラミジア-トラコマティス感染症の意義" 日本医師会雑誌. 109. 1933-1940 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 滝沢憲: "女性性器がんに対する抗癌剤の選択と卵巣機能に及ぼす影響" Onclology and Chemotherapy. 9. 11-15 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 滝沢憲: "妊娠に合併した子宮頭部高度異形成・上皮内癌および浸潤癌の細胞癌" 日本臨床細胞学会誌. 32. 900-905 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 滝沢憲: "腹腔内化学療法、AFIIとSTS併用によるCDDP腹腔内化学療法の副作用軽減と効果増強" Oncology and Chemotherapy. 9. 308-312 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 滝沢憲: "知っておきたい婦人科腫瘍の診断治療、治療方針と予後、卵巣悪性腫瘍" Medical Digest. 42. 18-22 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 原田誠: "進行卵巣癌に対するNeoddjiuvant Chemotherapyの効果" Oncology and Chemotherapy. 9. 250-258 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 滝沢憲: "女性の病気、月経の異常、In家兎の医学" 保健同人社, 1443-1452 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 滝沢憲: "がん化学療法の副作用対策ハンドブック" 先端医学社, 197-205 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 滝沢 憲: "Angiotensin II 併用 Cisplatin‐Sodiumthiosulfate 二経路化学療法の臨床・薬物動態的検討" 日本産婦人科学会誌. 44. 595-602 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 滝沢 憲: "抗癌剤の副作用軽減法23.生殖機能障害" 臨床婦人科・産科. 46. 1228-1230 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 滝沢 憲: "子宮頸癌患者における血清SCC,CEA値の臨床的有用性の検討" Oncology and Chemotherapy. 8. 373-379 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 滝沢 憲: "女性性器がんに対する抗癌剤の選択と卵巣機能に及ぼす影響" Oncology and Chemotherrpy. 9. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 原田 誠: "卵巣癌リンバ節転移の予後に及ぼす影響.最近7年間の治療成績より" 日癌治誌. 27. 1418 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松代 直美: "実験移植腫瘍におけるアドリアマイシン(ADM)投与時のエネルギー代謝動的分析の試み" 日本癌学会総会記事. 451 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 滝沢 憲: "がん化学方法の副作用対策・13脱毛とその対策" 先端医学社, 325-335 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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