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Basic mechanism of caloric response using isolated frog labyrinth

Research Project

Project/Area Number 04671042
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

SUZUKI Mamoru  Hiroshima University School of Medicine Associate Professor, 医学部, 助教授 (80116607)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsCaloric response / Semicircular canal / Action potential / Utricle / Ototoxic drug / Thermocomvective flow / Bull frog / 膜迷路 / 膨大部神経 / 内リンパ流動
Research Abstract

Basic mechanism of caloric response was studied using isolated bull frog semicircular canal and utricle. First, the membranous labylinth was en bloc removed to study dynamic transduction of the endolymphatic flow. When the anterior canal was stimulated, an excitatory action potential was evoked from the anterior amullary nerve. At the same time, an inhibitory response was induced through the posterior nerve. Therefore, the endolymphatic flow applied to a part of the labyrinth leads to a systemic fluid shift in other organ through dynamic transduction. When an aminoglycoside drug was added to the perilymphatic space of the posterior semicircular canal, the action potential was inhibited. Therefore, ionic environment of the perilymph is essential for maintaining a function of the vestibular sensory cell. Finally, the semicircular canal and utricle were stimulated by thermoconvection, since caloric response is induced by thermoconvective fluid flow in the endolymph. Frozen Ringer's solution was used to produce thermoconvective flow along the gravitational plane. When a semicircular canal was stimulated by thermoconvection, excitatory and inhibotory responses were observed according to the direction of the thermoconvection. Therefore, thermoconvective flow serves as a valid mechanical stimulus to the sensory cells. When the utricle was stimulated by thermoconvection with different directions, the responses were the same regardless of the stimuli. This indicates that the utricle has an equal directional excitability, thus might be most influenced by weightless condition.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 平川治男: "内外リンパ液aminoglycosideの半規管膨大部神経電位に及ぼす影響" Otdogy Japan. 3. 68 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 鈴木衞: "対流刺激による卵形嚢活動電位の反応方向性について" 耳鼻咽喉科臨床. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mamoru Suzuki: "Directional excitability of the utricle" ORL. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M., Suzuki: "Directional excitability of the utricle evoked by thermoconvection." Practica Otologica. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M., Suzuki: "Directional excitability of the utricle : A study by thermoconvective stimulation." ORL. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H., Hirakawa: "Effect of aminoglycosides on ampullary potential." Otology. 68 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 平川治男: "内外リンパ液aminoglycosideの半規管膨大部神経電位に及ぼす影響" Otology Japan. 3. 68 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 鈴木 衞: "対流刺激による卵形嚢活動電位の反応方向性について" 耳鼻咽喉科臨床. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] Mamoru Suzuki: "Directional excitability of the utricle" ORL. (印刷中).

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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