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Study of pathogenesis of immune mediated inner ear disease

Research Project

Project/Area Number 04671054
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionNippon Medical School

Principal Investigator

TOMIYAMA Shunichi  Nippon Medical School, Otolaryngology, Associate Professor, 医学部, 助教授 (00094665)

Co-Investigator(Kenkyū-buntansha) YAMANOBE Sigeharu  Nippon Medical School, Otolaryngology, Assistant Professor, 医学部, 講師 (20210510)
AOKI Hideharu  Nippon Medical School, Otolaryngology, Assistant Professor, 医学部, 講師 (80159293)
後藤 裕一  日本医科大学, 医学部, 助手
池園 哲朗  日本医科大学, 医学部, 助手
野中 学  日本医科大学, 医学部, 助手
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
KeywordsInner ear / Endolymphatic sac / Immune response / Attack of vertigo / Endolymphatic hydrops / Sensory hearing loss / Caloric response / Radical oxygen / 〓〓難聴 / 内耳免疫 / 蝸電図 / 聴性脳幹反応 / 自発眼振 / 外リンパ抗体
Research Abstract

This study investigated pathophysiology of inner ear immune-injury in the animal model. Guineapigs were systemically presensitized with keyhole limpet hemocyanin (KLH) and were directly challenged with KLH to the endolymphatic sac (ES). Endolymphatic hydrops (EH) appeared in the saccule and the basal turn as soon as 5 hours and rapidly developed within 2 days. After one week, EH gradually disappeared. However, after 9 weeks, EH reccurred in the saccule, basal and apical turn. Cellular reaction in the ES obviously ocurred from 5 hours and reached maximum at 12 hours. These cells were acute inflammatory cells. After one week, lymphocytes and plasma cells were prodominant and remained for more than 3 months. In the acute inflammatory phase, hemorrhage occasionally occured mainly in the perilymphatic region in which case degeneration of sensory cells often accompanied. Myeloperoxidase distributed in the ES, sensory hair cells, spiral ligament and stria vasularis as soon as 5 hours and reached maximum within one day. Irritative spontaneous nystagmus occurred nearly after 12 hours and paralytic nystagmus followed nearly for 48 hours. Elevation of hearing threshold as well as suppression of caloric response occurred after 2 days and thereafter recoverd in most animals. Elevation in perilymphatic anti-KLH antibody levels within one week correlated well to the severity of morphophsyiological damage of the inner ear. These results suggested that recurrent immune reaction in the ES may lead to cause attack of vertigo, similar to Meniere's disease.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] S.Tomiyama: "The development of endolymphatic hydrops following local immune response in guinea pigs" Acta Otolaryngol(Stockh). 112. 470-478 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 池園哲郎: "内リンパ嚢局所免疫動物の蝸電図" 日耳鼻. 95. 809-816 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 後藤裕一: "内リンパ嚢局所免疫反応による内耳障害-聴性脳幹反応への影響-" 日耳鼻. 95. 963-968 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 富山俊一: "メニエール病と内耳免疫" 耳喉頭頚. 64. 891-895 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Tomiyama: "Endolymphatic hydrops induced by immune response of the endolymphatic sac;it's relation to the perilymph antibody levels" Ann Otol Rhinol Laryngol. 101 Suppl 157. 48-53 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Nonaka: "Vestibular disorder following immune response of the endolymphatic sac in the ginea pig." Ann Otol Rhinol Laryngol. 101 Suppl 157. 54-57 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 富山俊一: "内リンパ嚢局所免疫反応-内リンパ水腫の長期観察-" 日耳鼻. 95. 1906-1913 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 富山俊一: "内リンパ嚢局所免疫反応-反応初期における蝸牛組織障害" 日耳鼻. 96. 394-402 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 富山俊一: "内リンパ嚢局所免疫反応-外リンパ特異的抗体価と前庭感覚細胞変性との関係" 日耳鼻. 96. 745-750 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 富山俊一: "内リンパ嚢局所免疫反応による前庭機能異常-外リンパ抗体価と自発眼振との関係-" Equilibrium Res. Suppl 9. 144-147 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 坂上雅史: "実験的内リンパ水腫における血管条毛細血管の透過性" Equilibrium Res. 52. 186-191 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Tomiyama: "Immunological pathogenesis of the endolymphatic hydrops and its relation to Meniere's disease" Scanning Microscopy. 7. 907-920 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Tomiyama: "Immunological approach to Meniere's disease:Vestibular immune injury following immune reaction of the endolymphatic sac" ORL. 56. 11-18 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 北南和彦: "モルモット内リンパ嚢局所免疫における基底膜構成成分の変化" 日耳鼻. 97. 226-232 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kosaka K: "Inner ear autoantibodies in patients with sensorineural hearing loss" Acta Otolaryngol(Stockh). (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Tomiyama: "Immunobiology in Otology,Rhinogology and Laryngology" BF McCave,JE Veldman,G Mogi Kugler publication,Amsterdam/New york., 405 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Tomiyama: "Meniere's disease,Pathology,pathophysiology,diagnosis and treatment in press" R.Filipo,Kugler & Ghendini,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: "The development of endolymphatic hydrops following following local immune response in guinea pigs" Acta Otolaryngol(Stockh). 112. 470-478 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ikezono T: "Effects of inner ear immune response on auditory function in guinea pig" J Otolarygol Jpn. 95. 809-816 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Gotho Y: "Immuno-injury to the inner ear auditory system following secondary endolymphatic immune response" J Otolarygol Jpn. 95. 963-968 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: "Meniere's disease and inner ear immune response" Jibi Inkoka, Tokeibu Geka. 64. 891-895 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: "Encolymphatic hydrops induced by immune response of the endolymphatic sac ; it's relation to the perilymph antibody levels" Ann Otol Rhinol Laryngol. 101, suppl 157. 48-53 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nonaka M: "Vestibular disorder following immune response of the endolymphatic sac in the ginea pig" Ann Otol Rhinol Laryngol. 101, suppl 157. 54-57 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: "Devekionebt of endolymphatic hydrops following immune response in the endolymphatic sac of guinea pigs - Long term observation-" J Otolaryngol Jpn. 95. 1906-1913 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: "Cochlear immune injury following direct antigen challenge to the endolymphatic sac" J Otolaryngol Jpn. 96. 394-402 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: "Vestibular immune injuray related to perilymph antigen specific antibody levles in the guinea pig" J Otolaryngol Jpn. 96. 745-750 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: "Vestibular disorder followoing immune reaction in the endolymphatic sac- Relation of spontaneous nystagmus to perilympha antibody levels-" Eqwuil ibrium Res. suppl 9. 144-147 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sakagami M: "Vascular permeability of the stria vascularis in two models of experimental endolymphatic hydrops" Equilibrium Res. 52. 186-191 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: "Immunological pathogenesisi of endolymphatic hydrops and its relation to Meniere's disease" Scanning Miscoscopy. 7. 907-920 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: "Immunological approach to Meniere's disease : Vestibular immune injury following immune reaction of the endolymphatic sac" ORL. 56. 11-18 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hokunan K: "Alteration of the distribution of basement membrane components after secondary inner ear immune reaction in the guinea pig" J Otolaryngol Jpn. 97. 226-232 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: Immunobiology in Otology, Rhinogology and Laryngology. BF McCave, JE Veldman, G Mogi Kugler publication, 405 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomiyama S: Meniere's disease, Pathology, Pasthophysiology, Diagnosis and Treatment in press. R.Filipo, Kugler & Ghendini, (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 富山俊一: "内リンパ嚢局所免疫反応-反応初期における蝸牛組織障害" 日 耳鼻. 96. 394-402 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 富山俊一: "内リンパ嚢局所免疫反応-外リンパ特異的抗体価と前庭感覚細胞変性との関係" 日 耳鼻. 96. 745-750 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 富山俊一: "内リンパ嚢局所免疫反応による前庭機能異常-外リンパ抗体価と自発眼振との関係" Equilibrium Res. Suppl 9. 144-147 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 坂上雅史: "実験的内リンパ水腫における血管条毛細血管の透過性" Equilibrium Res. 52. 186-191 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Tomiyama: "Immunological pathogenesis of the endolymphatic hydrops and its relation to Meniere's disease" Scanning Microscopy. 7. 907-920 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Tomiyama: "Immunological approach to Meniere's disease: Vestibular immune injury following immune reaction of the endolymphatic sac" ORL. 56. 11-18 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 北南和彦: "モルモット内リンパ嚢局所免疫反応による基底膜構成成分の変化" 日 耳鼻. 97. 226-232 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kosaka K: "Inner ear autoantibodies in patients with sensorineural hearing loss" Acta Otolaryngol(Stockh). (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Tomiyama: "Meniere's disease,Pathology pathophysiology,diagnosis and treatment. in press" R.Filipo,Kugler & Ghendini,

    • Related Report
      1993 Annual Research Report
  • [Publications] 池園 哲郎: "内リンパ嚢局所免疫動物の蝸電図" 日本耳鼻咽喉科学会会報. 95. 809-816 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 後藤 祐一: "内リンパ嚢局所免疫反応による内耳障害 -聴性脳幹反応への影響-" 日本耳鼻咽喉科学会会報. 95. 963-968 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 富山 俊一: "内リンパ嚢局所免疫反応による内リンパ水腫 -長期観察-" 日本耳鼻咽喉科学会会報. 95. 1906-1913 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shunichi Tomiyama.: "Development of Endolymphatic Hydrops Following immuneresponse in the enoolymphatic sac of the guineapig" Acta Otolaryngol (stockh). 112. 470-478 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shunichi Tomiyama: "Endolymphatic hydrops induced by immune response of the endolymphalic sac:Relatiou to perilymph antibody levels" An wals of otology.Rhinology & Laryugology. 101 suppl.152. 48-53 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Manabu Nonaka.: "Vestibular disorders following immune response of the endolywphatic sac in the guineapig" Aunals of otology,Rhindogy & Laryugology. 101 補157. 54-57 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shwnichi Tomiyama: "Immunobiology in otology,Rhinology and Laryngology." Kugler Publications. B‐F・McCabe.J・E.Veldman and G.urogi., 407 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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