| Project/Area Number |
04671068
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Yamanashi Medical College |
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Principal Investigator |
YAMABAYASHI Shigeki Yamanashi Medical College, Department of Ophthalmology, Assistant Professor, 医学部, 講師 (90126714)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Quick-Freezing and Deep-Etching / Replica / Glaucoma / Collagen / Polyethyleneimine / Proteoglycan / Alcian Blue / High Iron Diamine / 急速凍結ディープエッチング / 高鉄ジアミン染色 / アルシアンブルー染色 / PAS染色 / ポリエチレンイミン染色 / 高眼圧モデル / 急速凍結ーディープエッチング法 |
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| Research Abstract |
Histochemical and ultracytochemical studies on optic disc changes by experimental glaucoma were carried out. Experimental glaucoma were done by means of trabecular irradiation with argon lasor. Optic discs were ressected after constant pressure fixation with paraformaldehyde, also fixed with 0.25% glutaraldehyde, quick-freezed in a mixture of propane and isopentane. Replica was made by rotary shadowing with platinum and carbon. Light microscopic materials with some hisochemical staining and quick-freezing deep-etching materials with polyethyleneimine reaction were also carried out for the present investigation. Quick-freezing and deep-etching showed a decreasing of number of collagen fibers at lamina cribrosa in experimental groups. Polyethyleneimine reaction showed many positive materials over collagen fibers. In AB(pH 1.0) and HID staining, low intensity in color was shown in experimental groups. From the results obtained, it was speculated that number of collagen fibers and amount of proteoglycan were reduced by increasing of intraocular pressure.
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