| Project/Area Number |
04671101
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
TAKAHASHI Hiroshi NAGASAKI UNIVERSITY ORAL PATHOLOGY ASSOCIATE PROFESSOR, 歯学部, 助教授 (20124597)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Shuichi NAGASAKI UNIVERSITY ORAL PATHOLOGY ASSISTANT DOCTOR, 歯学部, 助手 (00181355)
HARA Yoshitaka NAGASAKI UNIVERSITY PERIODONTOLOGY ASSOCIATE PROFESSOR, 歯学部, 助教授 (60159100)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Gingiva / Periodontitis / RT-PCR method / mRNA / Cytokine / 細胞接着分子 / 受容体系遺伝子 |
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| Research Abstract |
The mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR) method and immunohistochemistry of cytokine were studied with gingival biopsies from 20 cases of adult periodontitis and 4 cases of rapidly progressive periodontitis. Twenty-four cases were divided into two groups of mild type and severe type, which were defined by depth of periodontal pocket. The depth of periodontal pocket which is less than 6 mm is the feature of mild periodontitis. The severe periodontitis is characterized by the increased depth of periodontal pocket (more than 6 mm).
Immunohistochemical study showed that immunocompetent cells in mild periodontitis reacted with interleukin 2,3 and 4. By contrast, interleukin 1alpha, 1beta, 2, 4 and 6 positive cells were frequently observed in severe periodontitis. By RT-PCR method, mRNA expression of interleukin 1beta in severe periodontitis was significantly higher than that of interleukin 1alpha in severe periodontitis.
Expression of mRNA of interleukin 2, 3, 4, 5 and 6 was not detected in the inflamed gingiva.
These results suggest that interleukin 1beta may play the important role of pathogenesis and chronic status.
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