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Comparison between mRNA expression by RT-PCR method in human gingiva of periodontitis and clinical finding

Research Project

Project/Area Number 04671101
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionNagasaki University

Principal Investigator

TAKAHASHI Hiroshi  NAGASAKI UNIVERSITY ORAL PATHOLOGY ASSOCIATE PROFESSOR, 歯学部, 助教授 (20124597)

Co-Investigator(Kenkyū-buntansha) FUJITA Shuichi  NAGASAKI UNIVERSITY ORAL PATHOLOGY ASSISTANT DOCTOR, 歯学部, 助手 (00181355)
HARA Yoshitaka  NAGASAKI UNIVERSITY PERIODONTOLOGY ASSOCIATE PROFESSOR, 歯学部, 助教授 (60159100)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsGingiva / Periodontitis / RT-PCR method / mRNA / Cytokine / 細胞接着分子 / 受容体系遺伝子
Research Abstract

The mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR) method and immunohistochemistry of cytokine were studied with gingival biopsies from 20 cases of adult periodontitis and 4 cases of rapidly progressive periodontitis. Twenty-four cases were divided into two groups of mild type and severe type, which were defined by depth of periodontal pocket. The depth of periodontal pocket which is less than 6 mm is the feature of mild periodontitis. The severe periodontitis is characterized by the increased depth of periodontal pocket (more than 6 mm).
Immunohistochemical study showed that immunocompetent cells in mild periodontitis reacted with interleukin 2,3 and 4. By contrast, interleukin 1alpha, 1beta, 2, 4 and 6 positive cells were frequently observed in severe periodontitis. By RT-PCR method, mRNA expression of interleukin 1beta in severe periodontitis was significantly higher than that of interleukin 1alpha in severe periodontitis.
Expression of mRNA of interleukin 2, 3, 4, 5 and 6 was not detected in the inflamed gingiva.
These results suggest that interleukin 1beta may play the important role of pathogenesis and chronic status.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Hiroshi Takahashi: "Immunophenotypic analysis of extranodal non-Hodgkin's lymphomas in the oral cavity." Pathology,Research and Practice. 189. 300-311 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Pe MB: "Ultrastructural demonstration of rat myofibroblasts in phenytoin-induced gingival overgrowth." Japan Journal of Oral Biology. 35. 496-504 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 金子高士・原宜興・岩崎由佳・市丸英二・岩永正憲・加藤伊八: "歯周炎歯肉における抗ヒトIgG抗体保有細胞の存在に関する免疫組織学的研究" 日本歯周病学会会誌. 35. 236-242 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 井村憲二,原宜興,加藤伊八: "内毒素による歯肉の炎症と破骨細胞形成への免疫系の関与についての病理組織学的研究" 日本歯周病学会会誌. 35. 625-637 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Takahashi: "Nucleolar organizer regions(NORs) in lymphoproliferative disorders of the human salivary gland." Analytical Cellular Pathology. 6. 51-63 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Takahashi: "Estimation of Silver-Binding Nucleolar Organizer Regions(AgNORs) in Lymphoproliferative Disorders of Gastro-intestinal Tract." Pathology,Research and Practice. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Takahashi, Shuichi Fujita, Haruo Okabe, Nobuo Tsuda, Fumiaki Tezuka: "Immunophenotypic Analysis of Extranodal Non-Hodgkin's Lymphomas in the Oral Cavity." Pathology, Research and Practice. 189. 300-311 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Pe MB, Sano K, Fujita S, Inokuchi T: "Ultrastructural demonstration of rat myofibroblasts in phenytoin-induced gingival overgrowth." Japan Journal of Oral Biology. 35. 496-504 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Takahashi, Shuichi Fujita, Jun Cheng, Nobuo Tsuda, Fumiaki Tezuka, Ai-Ru Liu, Haruo Okabe: "Nucleolar organizer regions (NORs) in lymphoproliferative disorders of the human salivary gland." Analytical Cellular Pathology. 6. 51-63 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takashi Kaneko, Yoshitaka Hara, Yuka Iwasaki, Eiji Ichimura, Masanori Iwanaga, Ihachi Kato: "Immunohistological Study of Plasma Cells Bearing Immunoglobulins Reactive to Human IgG in Human Periodontitis." Journal of the Japanese Society of Periodontology. 35. 236-242 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kenji Imura, Yoshitaka Hara, Ihachi Kato: "A Histological Study on Participation of the Immune System in gingival Inflammation and Osteoclastic Formation Induced by Endotoxin Derived from Escherichia coli." Journal of the Japanese Society of Periodontology. 35. 625-637 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakajima M, Sato H, Noda N, Uji Y, Taen A, Takahashi H, Okabe H: "A Case of Plasmacytoma Arising from Mandible." Journal of Kyushu Dental College. 47. 297-303 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Takahashi, Shuichi Fujita, Haruo Okabe, Nobuo Tsuda, Fumiaki Tezuka: "Estimation of Silver-Binding Nucleolar Organizer Regions (AgNORs) in Lymphoproliferative Disorders of Gastrointestinal Tract." Pathology, Research and Practice. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Takahashi: "Immunophenotypic analysis of extranodal non-Hodgkin's Iymphomas in the oral cavity" Pathology,Research and practice. 189. 300-311 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Pe MB: "Ultrastructural demonstration of rat myofibroblasts in phenytoin-induced gingival overgrowth." Japan Journal of Oral Biology. 35. 496-504 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiroshi Takahashi: "Nucleolar organizer regions (NORs) in lymphoproliferative disorders of the human salivary gland" Analytical Cellular Pathology. 6. 51-63 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 金子高士・原宜興・岩崎由佳・市丸英二・岩永正憲・加藤伊八: "歯周炎歯肉における抗ヒトIgG抗体保有細胞の存在に関する免疫組織学的研究" 日本歯周病学会会誌. 35. 236-242 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 井村憲二,原宜興,加藤伊八: "内毒素による歯肉の炎症と破骨細胞形成への免疫系の関与についての病理組織学的研究" 日本歯周病学会会誌. 35. 625-637 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiroshi Takahashi: "Estimation of Silver-Binding Nucleolar Organizer Regions (AgNORs) in Lymphoproliferative Disorders of Gastrointestinal Tract" Pathology,Research and practice22GD06:(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiroshi Takahashi: "Distribution of tissue markers in acinic cell carcinomas of salivary gland." Pathology,Research and Practice. 188. 692-700 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroshi Takahashi: "Primary malignant lymphoma of the salivary gland:a tumor of mucosa-associated lymphoid tissue." Journal of Oral Pathology and Medicine. 21. 318-325 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shuichi Fujita: "Nucleolar organizer regions in malignant salivary gland tumors." Acta Pathologica Japonica. 42. 483-486 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shuichi Fujita: "Distribution of natural killer cells in periodontal diseases:An immunohistochemical study." Journal of Periodontology. 63. 686-689 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shuichi Fujita: "Proliferative activity in normal selivary gland and pleomorphic adenoma:A study by argyrophilic nucleolar organizer region (AgNOR) staining." Acta Pathologica Japonica. 42. 573-578 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroshi Takahashi: "Immunophenotypic analysis of extranodal non-Hodgkin's lymphoma in the oral cavity." Pathology,Research and Practice.

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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