| Project/Area Number |
04671107
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Meikai University, School of Dentistry |
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Principal Investigator |
HANAWAWA Shigemasa Meikai University, School of Dentistry associate Professor, 歯学部, 助教授 (60060258)
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHITA Akira Meikai University, School of Dentistry associate Professor, 歯学部, 助手 (70236454)
AMANO Shigeru Meikai University, School of Dentistry associate Professor, 歯学部, 講師 (90167958)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Periodontal tissues / Cytokine / Gene expression / Signal transduction / Network mechanism / 骨芽細胞 / TGT-beat / 単球走化性因子 / Pgingivalis / 歯周ポケット |
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| Research Abstract |
We have previously demonstrated that lipopolysaccharide and finfriae derived from periodotal pathogens induces strongly gene expression of several inflammatory cytokines in cell components of periodontal tissues.
Recently, many studies have shown that IL-1, TNF-alpha, and IL-6 werepotent stimulater for bone resortptoon. Therefore, these cytokines may play an important role in initiation and development of adult periodontal disease which is characterized in inflammation of gingiva and alveolar bone resorption.
In the present study, we studiesd gene expression of inflammaytory cytokines of gingival fibroblasts and osteoblastsic cells treated by cytokine, and further signal transducing mechanism of their gene expressions. The following results was obtaind in this study. 1) Marked gene expression of monocyte chemoattractant MCP-1 was observed in gingival tissues of adult periodontal patients. 2) TNF-alpha induces expression of monocyte chemoattractant JE/MCP-1 via fos and jun genes in mouse osteoblastic cells MC3T3-E1 cells. 39) IL-1 induces expression of monocyte chemoattractant JE/MCP-1 in mouse osteoblastic cells and human gingival fibroblasts.
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