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Functional and morphological analysis of the main excretory duct IV.

Research Project

Project/Area Number 04671115
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionFukuoka Dental College

Principal Investigator

SATO Atsuko  Fukuoka Dental college, Oral Anatomy, Associate prof., 歯学部, 助教授 (20099047)

Co-Investigator(Kenkyū-buntansha) SATO Atsuko  Fukuoka Dental college, Oral Anatomy, Associate prof. (20099047)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsTuft cell / Main excretory duct / Submandibular gland / Glycoconjugate cytochemistry / Lectins / ruthenium red / Postnatal development / Male rat / ルテニユ-ム レッド / Submandibular gland / Main excretory duct / Tuft cells / postnatal development / Rat(Wister strain) / PA-TCH-SP-PD / Glycoconjugate cytochemistry / Rat(Wistar) / Tuft cell / Microvilli
Research Abstract

The tuft cells found in the main excretory duct epithelium of the submandibular gland are also present in the hollow organs of endodermal orgin such as the respiratory systems and the alimentary systems. Their common chracteristics are the finger-like long microvilli, microtubules and microfilaments running parallel to the cell axis, and a lot of vesicles and tubules in the apical cytoplasm. However, their functions are still unknown. The following experiments were done to establish their functions
1)Vesicles and tubules contained the filamentous substance. Glycoconjugate cytochemistry were performed to prove the character of the filamentous substance. Vesicles, tubules Golgi elements and plasma membrane of the microvilli stained positive.
2)The tuft cells were studied by embedding water soluble resin LRW and postembedded staining with lectin/ colloidal-gold (CG) conjugate-Con-A,PNA,SBA,UEA-1, DBA.Con-A was observed in the vesicles and tubules in the apical cytoplasm.
3)The postnatal development of tuft cells at various stages, 0,1,7,14,21,28 and 54 days, was investigated by scanning and transmission electron microscopy. Tuft cells were already recognized at birth. Their number reached approximately 6% of the total epithelial cells. The frequency of tuft cells in the adult stage was about 7%. However, microvilli were smaller than the adult ones. In fine structure, the tuft cells at 28 days after birth reached the same level as that at birth.
Conclusion
The tuft cells in the main excretory duct epithelium of the rat submandibular gland seem to engage in secretory activity of glycoconjugates, which may be related to the reception because of long microvilli protruding into the lumen.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 佐藤敦子,三好作一郎: "唾液腺主導管上皮の超微形態学" 電子顕微鏡. 28. 122-127 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato,A.: "Fine structure of the tuft cells in the main excretory duct of the rat submandibular gland." Cell & Tissue Research. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato,A.: "Postnatal development of the tuft cells of the rat submandibular gland." Anatomical Record. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, A.: "Fine structure of the tuft cells in the main excretory duct of the rat submandibular gland." Cell Tissue Res. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, A.: "Postnatal development of the tuft cells of the rat submandibular gland." Anatomical Record. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 佐藤敦子、三好作一郎: "唾液腺主導管上皮の超微形態学" 電子顕微鏡. 28. 122-127 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,A.: "Fine structure of The tuft cells inthe main excretory duct of the rat submandibular gland." Cell Tissue Res. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤敦子: "唾液腺主導管上皮の超微形態学" 電子顕微鏡. 28. 122-127 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato A.: "Postnatal development of the tuft cells in the main excretory duct epithelium of the submandibular gland of the rat." Cell Tissue Res. (発表予定).

    • Related Report
      1993 Annual Research Report
  • [Publications] Atsuko SATO: "Fine structure and subepithelial capillaries of the main excretory duct epithelium of the submandibular gland of the japanese monkey,Macaca fuscara." Anatomical Record. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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