Functional and morphological analysis of the main excretory duct IV.
| Project/Area Number |
04671115
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
SATO Atsuko Fukuoka Dental college, Oral Anatomy, Associate prof., 歯学部, 助教授 (20099047)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Atsuko Fukuoka Dental college, Oral Anatomy, Associate prof. (20099047)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Tuft cell / Main excretory duct / Submandibular gland / Glycoconjugate cytochemistry / Lectins / ruthenium red / Postnatal development / Male rat / ルテニユ-ム レッド / Submandibular gland / Main excretory duct / Tuft cells / postnatal development / Rat(Wister strain) / PA-TCH-SP-PD / Glycoconjugate cytochemistry / Rat(Wistar) / Tuft cell / Microvilli |
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| Research Abstract |
The tuft cells found in the main excretory duct epithelium of the submandibular gland are also present in the hollow organs of endodermal orgin such as the respiratory systems and the alimentary systems. Their common chracteristics are the finger-like long microvilli, microtubules and microfilaments running parallel to the cell axis, and a lot of vesicles and tubules in the apical cytoplasm. However, their functions are still unknown. The following experiments were done to establish their functions
1)Vesicles and tubules contained the filamentous substance. Glycoconjugate cytochemistry were performed to prove the character of the filamentous substance. Vesicles, tubules Golgi elements and plasma membrane of the microvilli stained positive.
2)The tuft cells were studied by embedding water soluble resin LRW and postembedded staining with lectin/ colloidal-gold (CG) conjugate-Con-A,PNA,SBA,UEA-1, DBA.Con-A was observed in the vesicles and tubules in the apical cytoplasm.
3)The postnatal development of tuft cells at various stages, 0,1,7,14,21,28 and 54 days, was investigated by scanning and transmission electron microscopy. Tuft cells were already recognized at birth. Their number reached approximately 6% of the total epithelial cells. The frequency of tuft cells in the adult stage was about 7%. However, microvilli were smaller than the adult ones. In fine structure, the tuft cells at 28 days after birth reached the same level as that at birth.
Conclusion
The tuft cells in the main excretory duct epithelium of the rat submandibular gland seem to engage in secretory activity of glycoconjugates, which may be related to the reception because of long microvilli protruding into the lumen.
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Report
(4 results)
Research Products
(10 results)
