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PHYSIOLOGICAL AND PATHOLOGICAL STUDIES OF PROTEINASES IN THE RAT TRIGEMINAL GANGLION CELLS

Research Project

Project/Area Number 04671125
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

NAKANISHI Hiroshi Ph.D.  KYUSHU UNIVERSITY FUCULTY OF DENTISTRY PHARMACOLOGY ASSISTANT PROFESSOR, 歯学部, 助手 (20155774)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Kenji Ph.D.  KYUSHU UNIVERSITY FUCULTY OF DENTISTRY PHARMACOLOGY PROFESSOR, 歯学部, 教授 (40091326)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsCATHEPSINS / TRIGEMINAL GANGLION / AGING / LYSOSOME / PHAGOCYTOSIS / 免疫組織 / 免疫電顕 / ラット
Research Abstract

The localizations and enzymatic activities of cathepsins B, D, and L, the most typical lysosomal proteinases, were determined by immunohistochemistry, enzymatic assay and immunoprecipitation in the trigeminal ganglion (TG) of young (2-3 months) and aged (28-31 months) rats. In the young rat, granular immunoreactive products of these enzymes showed homogeneous distributions through the cytoplasm. In the aged rat, however, immunoreactive products of these enzymes were found to be localized at a focal cytoplasmic site of most TG neuron forming coarse aggregates. At the immunoelectron microscopic level, immunoreactive products of cathepsin B were detected in mainly secondary lysosomes and partially primary lysosomes and residual bodies, which were accumulated at a focal perinuclear site of TG neurons from aged rat. Enzymatic activities of cathepsins B and L in the TG of aged rat were significantly lower than those of young rat, whereas cathepsin D activity was unchanged with aging. Cathepsin E, a non-lysosonmal aspartic proteinase, was not detected in TG neurons by either immunohistochemistry or enzymatic assay. The present study atrongly suggests that the focal accumulation of cathepsins B, L and D and the reduced activities of cathepsins B and L in the TG neurons of aged rat are closely linked with the increased autophagocytosis and the genesis of residual bodies and/or lipofuscins which are the most ubiquitous age-related cytological alteration. Thus, these enzymes, especially cathepsins B and L, can be used as markers of rate of aging as well as for increased phagocytosis in the TG neuronal system.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 天野功他: "老化に伴う中枢ならびに末梢神経細胞におけるCathepsin群の変動" 歯科基礎医学会雑誌. 34. 164 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 天野功他: "老化に伴う神経細胞障害とCathepsin群の関与" 歯科基礎医学会雑誌. 35. 114 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakanishi,H.et al.: "Transient forebrain ischemia induces increased expression and specific localization of cathepsins E and D in rat hippocampus and neostriatum" Experimental Neurology. 121. 215-223 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakanishi,H.et al.: "Age-related changes in activities and localizations of cathepsins D,E,B and L in the rat brain tissues" Experimental Neurology. 1-10 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] AMANO, T., NAKANISHI, H., OKA, M.AND YAMAMOTO, K.: "AGE-RELATED CHANGES OF CATHEPSINS IN THE CENTRAL AND PERIPHERAL NEURONS" JPN.J.ORAL BIOL.VOL.35, SUPPL.164 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] AMANO, T., NAKANISHI, H., OKA, M., AND YAMAMOTO, K.: "INVOLVEMENT OF CATHEPSINS IN AGE-RELATED NEURONAL DEGENERATION" JPN.J.ORAL BIOL.VOL.35, SUPPL.114 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] NAKANISHI,H., TSUKUBA, T., KONDOU, T, TANAKA, T.AND YAMAMOTO, K.: "TRANSIENT FOREBRAIN ISCHEMIA INDUCES INCREASED EXPRESSION AND AND SPECIFIC LOCALIZATION OF CATHEPSINS E AND D IN RAT HIPPOCAMPUS AND NEOSTRIATUM." EXPERIMENTAL NEUROLOGY. 121. 215-223 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] NAKANISHI, H., TOMINAGA, K., AMANO, T., HIROTSU, I, INOUE, I.AND YAMAMOTO, K.: "AGE-RELATED CHANGES IN ACTIVITIES AND LOCALIZATIONS OF CATHEPSINS D, E, B, AND L IN THE RAT BRAIN TISSUES." EXPERIMENTAL NEUROLOGY. 125. 1-10 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 天野 功他: "老化に伴う神経細胞障害とCathepsin群の関与" 歯科基礎医学会雑誌. 35. 114- (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakanishi,H.et al.: "Transient forebrain ischemia induces increased expression and specific localization of cathepsins E and D in rat hippocampus and neostriatum" Experimental Neurology. 121. 215-223 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakanishi,H.et al.: "Age-related changes in activities and localizations of cathepsins D,E,B and L in the rat brain tissues" Experimental Neurology. 125. 1-10 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 天野 功他: "老化に伴う中枢ならびに未梢神経細胞におけるCathepsin群の変動" 歯科基礎医学会雑誌. 34. 164- (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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