| Project/Area Number |
04671159
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Conservative dentistry
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
ARAI Hideo Okayama Univ.Dental Sch., Assistant, 歯学部, 助手 (70222718)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Keiso Okayama Univ.Dental Sch., 歯学部・附属病院, 助手 (70243475)
KOBAYASHI Yoshitomo Okayama Univ.Dental Sch., Assistant, 歯学部, 助手 (90234856)
KURIHARA Hidemi Okayama Univ.Dental Sch., Assistant Prof., 歯学部, 助教授 (40161765)
MURAYAMA Yoji Okayama Univ.Dental Sch., Professor, 歯学部, 教授 (50029972)
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | Periodontitis / Interleukin-2 / Intracellular signal transduction / T cell / CD3 / Interleukin-2(IL-2) / IL-2レセプター / 若年性歯周炎 / 細胞内Ca^<2+>濃度 / phorbol myristate acetate(PMA) / ionomycine / 細胞内情報伝達系 |
|---|
| Research Abstract |
The purpose of this study was to assess the role of interleukin-2 (IL-2) in the pathogenesis of periodontitis. The levels of IL-2 produced from mononuclear cells were measured after stimulating with a CD3 monoclonal antibody (CD3 mAb) in 45 patients (12 with juvenile periodontitis [JP], 20 with rapidly progressive periodontitis and 13 with adult periodontitis) and 20 healthy subjects (HS). Six subjects including 3 JP patients demonstrated elevated IL-2 level. When phorbol myristate (PMA) was substituted for monocytes, there existed one JP patient with significantly high IL-2 producing capacity, and two JP patients and one HS with low capacity. To investigate the cause of their unusual IL-2 producing capacities, intracellular signal transduction system in T cells was examined. The levels of IL-2 produced upon stimulation with ionomycin and PMA were proportionate to those produced upon stimulation with CD3 mAb and PMA in the subjects examined except one JP patient ; suggesting that the elevated IL-2 producing capacity of this patient is related to the intracellular calcium ion level after stimulation of CD3 molecules. These results suggest that IL-2 producing capacity is responsible for the pathogenesis of a certain type of early-onset periodontitis, and that intracellular signal transduction system is concerned with the imbalance of IL-2 production in some individuals.
|
