| Project/Area Number |
04671166
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Kagoshima University |
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Principal Investigator |
SETOGUCHI Takashi Kagoshima University, Dental School, Assistant, 歯学部, 助手 (60206646)
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| Co-Investigator(Kenkyū-buntansha) |
KAMADA Tetsuro Kasoshima University, Faculty of Medicine, Assistant, 医学部, 助手 (10204603)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Polymorphonuclear leukocyte / Diabetes mellitus / Superoxide / periodontitis associated bacteria / Lipopolysaccharide / Chemotaxis / Cytotoxicity |
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| Research Abstract |
The main cause of periodontal disease is bacteria in periodontal pocket. The host factors are also important in the progression of periodontal disease. The susceptibility to periodontal disease of diabetes mellitus (DM) patient is reported, but the mechanism is unknown. We examined the effects of lipopolysaccharide (LPS) from periodontal bacteria on Polymorphonuclear leukocytes (PMN) from DM patient.
We selected 20 DM patients and 16 healthy control. We examined
1) superoxide production by PMN stimulated with FMLP measured using chemiluminescence,
2) chemotaxis to FMLP using Boyden chamber methods,
3) membrane fluidity measured by fluorescence anisotropy technique. The superoxide production by PMN from DM patients was significantly higher than those from control. Chemotaxis and membrane fluidity were not significantly different. We extracted LPS from Porphyromonas gingivalis and Prevotella intermedia using hot water phenol methods. The LPS highly stimulated the superoxide production by PMN from DM patient than those from control. these results indicate the possibility that the superoxide produced by PMN affect the fibroblast in periodontal tissue.
We cultured gingival fibroblasts, stimulated the cells with PMN and LPS, and measured the release of LDH.The result was that the simultaneous stimulation of PMN and LPS was injurious to gingival fibroblasts. We will continue to examine which factor, superoxide, lysosomal enzyme or others, induce the change.
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