| Project/Area Number |
04671227
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
KAWAHARA Michio Hiroshima Univ. Dental Hosp.Prof., 歯学部・付属病院, 教授 (30034094)
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| Co-Investigator(Kenkyū-buntansha) |
KINUGAWA Hiroshi Hiroshima Univ.Dental Hosp.Assist., 歯学部・付属病院, 助手 (00177883)
藤井 宏融 広島大学, 医学部, 講師 (60034021)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Guinea pig / Isolated hepatocytes / Intracellular Ca^<2+> concentration / Halothane / Isoflurane / Sevoflurance / 細胞内CA^<2+>濃度 |
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| Research Abstract |
The effects of halothane on the intracellular Ca^<2+> concentration ([Ca^<2+>]i) of isolated guinea pig hepatocytes were investigated .When hepatocytes were incubated in Hank's solution containing Ca^<2+>, trypan blue uptake which indicates death of cell was 13.5<plus-minus>3.41%, and [Ca^<2+>]i was 158<plus-minus>73.5nM in control.By halothane exposure, both trypan blue uptake and [Ca^<2+>]i were increased in concentration-dependent fashion.When hepatocytes were incubated in Hank's solution excluded Ca^<2+>, trypan blue uptake was 31.2<plus-minus>6.40%, and [Ca^<2+>]i was 249<plus-minus>27.5nM in control.By halothane exposure, trypan blue uptake was increased in concentration-depandent fashion, while [Ca^<2+>]i was decreased.
From these results, the increases of trypan blue uptake in experiments 1 and 2 indicate the hepatotoxicity of halothane with or without Ca^<2+> in medium. The increase of [Ca^<2+>]i of hepatocytes in physiological condition indicate the direct injury of halothane to hepatocyte cell membrane.
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