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Studies on antitumor effect for Head and Neck tumor

Research Project

Project/Area Number 04671244
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionHINON UNIVERSITY

Principal Investigator

SUZUKI Kunio  School of Dentistry at Matsudo, Nihon University, professor, 松戸歯学部, 教授 (40119681)

Co-Investigator(Kenkyū-buntansha) 高須賀 文子 (中村 文子)  日本大学, 松戸歯学部, 助手 (10231474)
大峰 浩隆  日本大学, 松戸歯学部, 助手 (20213861)
福地 久和  日本大学, 松戸歯学部, 講師 (60228921)
SUZUKI Kunio  School of Dentistry at Matsudo, Minon University, professor (40119681)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
KeywordsColorectal cancer / Colon 26 / Experimental model / Adjuvant chemotherapy / 温熱療法 / 制癌剤 / 扁平上皮癌 / X線感受性 / 温熱感受性 / GSH量 / ADF量 / 温熱耐性 / ヒートショック蛋白質 / CHO細胞 / 術前化学療法(CAF変法) / 広背筋筋皮弁 / 高LET線(中性子線) / 生存率曲線 / 増感効果 / 頭頸部癌 / Human Tumor Clonogenic Assey / colony 形成能 / 細胞収量 / 感受性試験成巧率 / γ線生存曲線 / 温熱放射線増感効果
Research Abstract

We report an experimental model of surgery with adjuvant chemotherapy by a footpad transplantation-tumor-transplanted limb amputation method using Colon 26, a tumor derived from mouse colorectal cancer. Colon 26 cells at an inoculum of 5*16^6 cells/mouse were transplanted into the footpads of 6-week-old male CDF_1 mice. In the untreated group, all mice died of cachexia within a short period of time, as seen in subcutaneous transplantation. In a preliminary experiment, tumor-transplanted limbs were amputated, resulting in an improved survival with the occurrence of lung metastasis. Next, the time of amputation suitable for an experimental model of surgery with adjuvant chemotherapy was estimated by the following experiment. Colon 26 cells at an inoculum of 5*10^6 cells/mouse adjusted to a volume of 0.025 ml were transplanted into the footpads of mice on Day 0.Six mice were used in each experimental group. The transplanted limbs were amputated from Day 1 to Day 6, and the mice were observed for 60 days. Autopsies were performed to determine the causes of death. The 60-day survival curves of the mice with amputation on Day 5 or Day 6 were closest to the clinical postoperative survival curves for colorectal cancer in humans. The results obtained suggest that this was a suitable experimental model for surgery with adjuvant chemotherapy. All deaths of these experimental mice were due to lung metastasis.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 笠倉 雄一: "術前化学療法が泰功し、広背筋M-Cflapにて一期的根治術が施行し得た乳癌の一例" 埼玉県医学会雑誌. 28.3. 23-26 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小宮 正道: "口腔癌患者に対する皮膚移植" 日大口腔科学. 20.3. 436-441 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 馬嶋 秀行: "CHO細胞による温熱中性子線致死効果" 日本大学原子力研究所報告書. (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masasi FUJII,Mitugu KOCHI Kunio Suzuki, Takashi TANAKA: ""Experimental model of Surgery with Adjuvant chemotherapy for Colorectal Canser using Colon 26 Cells"-First report-" The Nihon univ. Journal of Medicin. Vol 36, No 5. 213-220 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 笠倉雄一: "術前化学療法が奏効し、広背筋MC-flapにて一期的根治術が施行し得た乳癌の一例" 埼玉県医学会雑誌. 28-3. 23-26 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 若松佳子: "異なる温度により誘導される温熱耐性とヒートショック蛋白質" 日大歯学. 67.5. 870-871 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 馬嶋秀行: "CHO細胞による温熱中性子線致死効果" 日大原子力研究所平成5年度報告書. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 馬嶋秀行: "細胞における温熱耐性の誘導とHSPの発現" 電子通信学会雑誌. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松本 裕子: "抗腫瘍剤 (bleomycin, peplomycin, 5‐fluorouracil, Cisplatin , carboplatin) 併用投与時のラットにおける障害性" 臨床薬理 23(I) 3月 1992年 (Jpn J Clin Pharmacol Ther). 23(1). 277-278 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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