| Project/Area Number |
04671364
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
ONOZAKI Kikuo Department of Hygienic Chemistry, Faculty of Pharmaceutical sciences Nagoya City University, Professor, 薬学部, 教授 (20101313)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Hidetoshi Department of Hygienic Chemistry, Faculty of Pharmaceutical sciences Nagoya City, 薬学部, 講師 (80198853)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | interleukin 1 / cell growth inhibition / melanoma / IL-1Ra / interleukin6 / IL-1 receptor / active oxygens / TNF / サイトカイン / マクロファージ |
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| Research Abstract |
A human melanoma cell line became resistant to the anti-proliferativr effect of human IL-1 apha after a long period of culture. Using the sensitive and resistant clones, we investigated the mechanism by which the cells acquired the resistance. Tesistant cells produced constitutively IL-1 alpha. The IL-1 induced IL-6 production in the resistant cells by an autocrine manner, and the resistant cells were also resistant to the anti-proliferative effect of IL-6. In one resistant clone, IL-6 receptor mRNA level was quite low, suggesting that the acquired resistance results from the lack of IL-6 receptor.
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