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FLUORESCENCE VITAL-MICROSCOPIC STUDY FOR CAPILLARY PERMEABILITY OF MACROMOLECULES

Research Project

Project/Area Number 04671388
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionThe University of Tokyo

Principal Investigator

SHIBATA Masahiro  TOKYO UNIV.MED SCH.LECTURER, 医学部(医), 講師 (60158954)

Co-Investigator(Kenkyū-buntansha) KAMIYA Akira  TOKYO UNIV.MED SCH.PROFESSOR, 医学部, 教授 (50014072)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsPERMEABILITY / MACROMOLECULES / VITAL-MICROSCOPY / CAPILLARY / MICROCIRCULATION / 骨格筋
Research Abstract

Diffusion and convection may be considered to be the main driving forces for the transcapillary exchange of macromolecules in the skeletal muscle. To determine these permeability parameters along a single capillary, we have newly developed a fluorescence vital-microscope, in which a pair of well-defined slit beams (28mum in width) were focused to cross at a chosen depth of the tissue region including only a single capillary. The beams excite the fluorescent tracer (FITC-Dextran, M.W.=40,000, 70,000 and 150,000) intensively at the crossing plane which is adjusted by the focal plane of the objective of the microscope. This microscope was applied to quantify the diffusive (P) and convective (V) components of the transcapillary movement of macromolecules independently. By applying this method to rabbit tenuissimus muscle under vasodilation, it was confirmed that convection is dominant to diffusion in the transcapillary exchange of macromolecules at all regions of the capillary. Furthermore, P values obtained in this study suggest that the capillary wall at the venular end has approximately 40 times more affinity to pass macromolecules than that at the true capillary.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] A.Kamiya,et al.: "Optimality analysis of vascular-tissue system for oxygen transport in mammal." J.Theoretical Biol.162. 229-242 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 柴田,高橋,神谷: "蛍光生体顕微鏡による巨大分子の毛細血管透過性計測法" 医用電子と生体工学. 31. 25-30 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 柴田,河村,神谷: "スリットレーザー蛍光生体顕微鏡を用いた実質臓器内微小循環の計測." 脈管学. 33. 1005-1009 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 柴田,神谷: "毛細血管における血流" 病理と臨床. 11. 776-783 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Shibata,M.Sohirad,A.Kamiya: "Heterogeneity of the capillary permeability to macromolecules." Microcirculation annual. 9. 175-176 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Kamiya,J.Ando: "Vascular endothelial cell functions and biomechanics" Endothelium. 1. 127-130 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Kamiya,M.Shibata,M.Sohirad(共著): "Vein:Functional role in the circulation" Springer-Verlag, 144 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Shibata and A.Kamiya: "Blood flow dependence of local capillary permeability of Cr-EDTA in the rabbit skeletal muscle." Jpn.J.Physiol.42. 631-639 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Shibata, T.Takahashi and A.Kamiya: "The mechanisms of transcapillary exchange of macromolecules." Microcirculation annual. 8. 149-150 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Kamiya, J.Ando: "Vascular endothelial cell functions and biomechanics." Endothelium. 1. 127-130 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Kamiya, et al.: "Optimality analysis of vascular-tissue system for oxygen transport in mammal." J.Theoretical Biol.162. 229-242 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Shibata, T.Kawamura, and A.Kamiya: "Microscopic measurements of local capillary permeability of macromolecules in the rabbit tenuissimus muscle." Jpn.J.BME. 31. 25-30 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Shibata, M.Sohirad, and A.Kamiya: "Heterogeneity of the capillary permeability to macromolecules." Microcirculation annual. 9. 175-176 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Kamiya, M.Shibata, and M.Sohirad: "Macromolecular permeability and hydraulic conductivity through large pore across a single venular capillary." Vein : Functional role in the circulation. Eds.by S.Hirakawa et al.23-32 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Kamiya,et al.: "Optimality analysis of vascular-tissue system for oxygen transport in mammal." J.Theoretical Biol.162. 229-242 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 柴田,高橋,神谷: "蛍光生体顕微鏡による巨大分子の毛細血管透過性計測法" 医用電子と生体工学. 31. 25-30 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 柴田,河村,神谷: "スリットレーザー蛍光生体顕微鏡を用いた実質臓器内微小循環の計測." 脈管学. 33. 1005-1009 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 柴田,神谷: "毛細血管における血流" 病理と臨床. 11. 776-783 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Shibata,M.Sohirad,A.Kamiya: "Heterogeneity of the capillary permeability to macromolecules." Microcirculation aannual. 9. 175-176 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Kamiya,J.Ando: "Vascular endothelial cell functions and biomechanics" Endothelium. 1. 127-130 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Kamiya,M.Shibata,M.Sohirad(共著): "Vein:Functional role in the circulation" Springer-Verlag, 144 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Shibata and A.Kamiya: "Blood flow dependence of local capillary permeability of Cr-EDTA in the rabbit skeletal muscle." Jpn.J.Physiol.42. 631-639 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Shibata,T.Takahashi,A.Kamiya: "The mechanisms of transcapillary exchange of macromolecules." Microcirculation aannual. 8. 149-150 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 柴田 政廣、高橋 龍尚、神谷 瞭: "蛍光生体顕微鏡による巨大分子の毛細血管透過性計測法." 医用電子と生体工学. 31. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 柴田 政廣、高橋 龍尚、神谷 瞭: "スリットレーザー蛍光生体顕微鏡を用いた実質臓器内微小循環の計測." 脈管学会誌. 33. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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