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In vitro binding of the carcinogen N-[methyl-^<14>C]-nitrosodimethylamine by algal dietary fibers and their role in reducing its bioaccumulation.

Research Project

Project/Area Number 04671397
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionKitasato University School of Hygienic Sciences

Principal Investigator

YAMAMOTO Ichiro  Kitasato University School of Hygienic Sciences Professor, 衛生学部, 教授 (10050361)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Hiroko  Kitasato University School of Hygienic Sciences Lecturer, 衛生学部, 講師 (50129269)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsalgal dietary fiber / binding of carcinogen / cellulose / polydextrose / bioaccumulation / radio-labeled carcinogen / N-[methyl-^<14>C]-nitrosodimethylamine / 水溶性食物繊維 / 非水溶性食物繊維 / 食用海藻 / 海藻抽出物 / あまのり / ほそめこんぶ / DMNA
Research Abstract

The first study was initiated to determine if algal dietary fibers (DF) bind the carcinogen N-[methyl-^<14>C]-nitrosodimethylamine ([^<14>C]-DMNA) in vitro and how bioaccumulation of orally-given carcinogen is affected by a diet containing algal DF.Eight kineds of algal DF, including powdered fronds of Laminaria religiosa (LRP) and agar (from Gracilaria verrucosa) were used in the in vitro test. Cellulose powder (CP) was used as a control DF.In vitro binding rates of DMNA by CP, LRP and agar were 0.28%, 0.65% and 0.21% of the initial dose, respectively. Rats fed a diet containing 2% LRP or 2% agar were examined at 3 h or 24 h after dosing. There was reduced retention of the orally-ingested DMNA in the liver, posibly because of reduced DMNA-adsorption from the intestinal tract earlier than 3 h after dosing. Binding rates of DMNA by algae were neither related to the DF values nor to the extent of reduction of DMNA-absorption from the intestinal tract.
The second study was performed to verify the effects of cellulose (nsoluble in water) and of polydextrose (soluble in water), in reducing bioaccumulation of the carcinogen [^<14>C]-DMNA in the same in vivo experimental system, using mice. As the results, [^<14>C]-bioaccumelation in CP-fed mice remarkably reduced at 1 h after dosing, compared with that in control mice.
In the time points later than 1 h after dosing, difference in the bioaccumulation between CP-fed mice and cntrols were decreased sowly as the time lapsed. The results suggest that absorption and metabolism of the carcinogen took lace within 1 h after dosing. As to polydextrosefed mice, there was no significant reductin of [^<14>C] uptake, except blood at 24 h after dosing.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] H.Maruyama & I.Yamamoto: "In vitro binding of the carcinogen N-[methyl-^<14>C]-nitrosodimethylamine by algal dietary fibers and their role in reducing its bioaccumulation." Journal of Applied Phycology. 5. 201-205 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Maruyama & I.Yamamoto: "In vitro binding of the carcinogen N-[methyl-^<14>C]-nitrosodimethylamine by algal dietary fibers and their role in reducing its bioaccumulation." Journal of Applied Phycology. 5. 201-205 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Maruyama & I.Yamamoto: "In vitro binding of the carcinogenN-[methyl-14C]-nitrosodimethylamine by algal dietary fibers and their role in reducing bioaccumulation" Journal of Applied Phycology. (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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