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Evaluation Of Clinical Laboratory Test By Using Quartz Crystal Resonator

Research Project

Project/Area Number 04671437
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionKumamoto University

Principal Investigator

OKABE Hiroaki  Kumamoto University, Medical School, Professor, 医学部, 教授 (20185466)

Co-Investigator(Kenkyū-buntansha) UJI Yoshinori  Kumamoto University, Medical School, Assistant, 医学部, 助手 (90203512)
OKAJIMA Kenji  Kumamoto University, Medical School, Instructor, 医学部, 講師 (60152295)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsQuartz Crystal Resonator / Oscillator / Blood Coagulation Factor / Fibrinogen / Thrambin / Piezoelectric Sensor / Viscosity
Research Abstract

Quantification of plasma fibrinogen concentration by quartz crystal viscosity sensor was investigated. At-cut crystal was used as viscosity sensor with a well-type cell in a liquid phase. The surface of quartz crystal was precoated with fibrinogen to protect the bonding of endogeneous fibrinogen in the plasma.
The assay was initiated by the addition of thrambin into reaction mixture.
The resonant frequency change was monitored by using a well-type cell, oscillator and frequency counter. The resonant frequency decrease due to the increase of viscosity by gelation of fibrinogen converted to fibrin.
The proposed method is simple, rapid, specific and accurate at the lower range.
Results obtained in proposed system showed good linearity over range 50-400 mg/dl. The comparison of proposed method with routine method used yielded a correlation coefficient ; gamma=0.702 and regression curve ; Y=1.89X(proposed method) - 110.21(n=20).
Reference interval of plasma fibrinogen concentration from healty adults has in the range of 170-390 mg/dl. Reproducibilities(CVs) was between 2.29% and 7.97% in plasma. The preliminary studies suggested that a quartz crystal resonator is useful for the measurement of gelation of plasma coagulation factors in the liquid phase.

Report

(2 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 宇治義則、岡部紘明、岡嶋研二、杉内博幸、中川富美子: "水晶振動子を用いた血液凝固因子の測定" 日本臨床化学会九州支部会誌. 4(校正中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岡部紘明、岡嶋研二、中川富美子、宇治義則、杉内博幸: "第13回臨床分析研究会要旨集「水晶振動子を用いた臨床検査法の開発」Vol.4" 臨床分析研究会事務局編(国立循環器病センター臨床検査部), 150 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshinori UJI et al: "Measurement of blood coagulation factors by quartz crystal resonator" Kyushu J.Clinical Chemistry. 4. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroaki OKABE et al: "Proceedings on XIII Conference of Clinical Analysis ; Evaluation of Clinical Laboratory Tests by Using Quartz Crystal Resonator." Committee of Clinical Laboratory Analysis. Vol.4. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 宇治義則、岡嶋研二他: "水晶振動子を用いた血液凝固因子の測定" 日本臨床化学会九州支部会誌. 4,(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岡崎紘明、岡嶋研二他: "第13回臨床分析研究会要旨集(水晶振動子を用いた臨床検査法の開発)" 臨床分析研究会事務局編(国立循環器病センター臨床検査部), 150 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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