|Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
1. Antigenicity of synthetic peptodes was evaluated by intcraction between anti-thyroglobulin antisera raised by immunization with human thyroglobulin and radioiodinated synthetic peptides which constitute hormonogenic tyrosines (HTg-1 (1-10), HTg-2 (2547-2558), HTg-6 (2737-2603) HTg-7 (2547-2571)) or control peptides (HTg-3 (2581-2591), Htg-4 (2592-2603), Htg-5 (2687-2694)). Among peptide with hormonogenic tyrosines HTg-2 showed binding of 11.5%(TG-1) and 19.9%(TG-2). binding with HTg-1, HTg-6, and HTg-7 were less than 10%. Thus high antigenicity of peptide which contain Tyr^<2553> was suggested.
2. We have tested blastogenesis of lymphocytes obtained from mice different H-2 haplotypes. High stimulation indices were obtained in mice straines of C57BL/6, BALB/c, B10BR, C3H, and CBA/j by HTg-1, HTg-2, HTg-6, and HTg-7 (SI,1.5-5.9). It was shown that peptide with hormonogenic tyrosines have high antigenicity at cellular level.
3. In order to examine the role of thyroid hormone autoantibodies on hypothalamo-pituitary-thyroid axis, rats were immunized with thyroglobulin and serum concentrations of rats TSH, T3, and T4 were measured. Despite extremely high T3 and T4 levels in rats, serum concentration of TSH stayd in the normal range. It was concluded that the presence of thyroid hormone antibodies does not affect hypothalamo-pituitary-tyhyroid axis.