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Analysis of thyroxine-binding globulin (TBG) gene regulation and inherited TGB abnormalities

Research Project

Project/Area Number 04671468
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionNagoya University

Principal Investigator

MORI Yuichi  Nagoya Uni. School of Medicine Assistant Prof., 医学部, 助手 (70230084)

Co-Investigator(Kenkyū-buntansha) OKAMOTO Hideki  Nagoya Uni. School of Medicine Medical staff, 医学部, 医員
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsThyroxine-binding globulin / Complete deficiency / Partial deficiency / Excess / Frameshift mutation / Gene screening / Intracellular transport / HNF-1 / TBG異常症 / 遺伝子解析 / クローニング / 転写調節
Research Abstract

1. Complete TGB deficiency in Japanese(TBG-CDJ)has a nucleotide deletion at codon 352 among 395 resulting in the production of C-terminal 22 amino acid truncation due to a frameshift and premature termination. The cDNA of TBG-CDJ was expressed in COS-1 cells. Pulse chase experiment revealed TBG-CDJ was retained in the rough endoplasmic reticulum resulting in complete deficiency in serum.
Gene screening showed 23 Japanese families with TBG-CD had a same mutation TBG-CDJ.
2. Abnormality of X-chromosome inactivation was found in a female TBG-CD patient. She was heterozygous of TBG-CDJ and normal TBG alleles. The latter allele was selectively inactivated.
3. Partial TBG deficiency in Japanese (TBG-PDJ) has a nucleotide substitution at codon 363 replacing normal pro (CCT) with leu (CTT). The TBG-PDJ was expressed in COS-1 cells. lt was revealed that delay of intracellular transport of TBG-PDJ was a cause of partial deficiency. Induction of GRP78, molecular chaperone, was found to participate the transport disorder.
4. Gene duplication was shown in a hereditary TBG excess family by using PCR and HPLC.
5. Human and rat genomic TBG genes were isolated and characterized. Promoter regions of both genes were sequenced and subcloned to CAT or luciferase reporter construct. HNF-1 (hepatocyte nuclear factor 1) binding site was found important fot basal and liver specific expression. Gene expression and serum. TBG were down regulated by corticosterone in rat.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Yoshitaka Hayashi: "Human thyroxine-binding globulin gene:complete sequence and transcriptional regulation." Molecular Endocrinology. 7. 1049-1060 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Charles H.Emerson: "Gene expression and serum thyroxine-binding globulin are regulated by adrenal status and corticosterone in the rat." Endocrinology. 133. 1192-1196 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ikuo Yamamori: "Gene screening of 23 Japanese families with complete thyroxine-binding globulin deficiency:identification of a nucleotide deletion at codon 352 as a common cause." Endocrine Journal. 40. 563-569 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshitaka Miura: "Sequence of a variant thyroxine-binding globulin(TBG)in a family with partial TBG deficiency in Japanese(TBG-PDJ)." Endocrine Journal. 40. 127-132 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsukasa Noguchi: "Hereditary complete thyroxine-binding globulin deficiency:identification by T3 resin uptake test and DNA analysis." Internal Medicine. 32. 6-9 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kambe Fukushi: "An additional carbohydrate chain in the variant thyroxine-binding globulin(TBG asp-96)impairs its secretion." Molecular Endocrinology. 6. 443-449 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshitaka Miura: "A truncated thyroxine-binding globulin due to a frameshift mutation is retained within the rough endoplasmic reticulum." The Journal of Clinical Endocrinology & Metabolism. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kyoko Takeda: "Gene screening in Japanese families with thyroxine binding globulin complete deficiency demonstrates that a nucleotide deletion at codon 352 may be a rase specific mutation." Clinical Endocrinology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 森 祐一: "TBG異常症とTBG遺伝子" Medicina. 31. 302-305 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 三浦義孝: "日本人のTBG完全欠損症(TBG-CDJ)の発症機序に関する研究" 名古屋大学環境医学研究所年報. 44. 161-165 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 妹尾久雄: "遺伝性サイロキシン結合グロブリン(TBG)異常症" ホルモンと臨床. 40. 1221-1227 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshitake Miura et al.: "A truncated thyroxine-binding globulin due to a frameshift mutation is retained within the rough endoplasmic reticulum" J Clin Endocrinol Metab. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshitake Hayashi et al.: "Human thyroxine-binding globulin gene : complete sequence and transcriptional regulation" Molecular Endocrinology. 7. 1049-1060 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Charles H.Emerson et al.: "Gene expression and serum thyroxine-binding globulin are regulated by adrenal status and corticosterone in the rat" Endocrinology. 133. 1192-1196 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ikuo Yamamori et al.: "Gene screening of 23 Japanese families with complete thyroxine-binding globulin deficiency : identification of a nucleotide deletion at codon 352 as a common cause" Endocrine Journal. 40. 563-569 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshitake Miura et al.: "Sequence of a variant thyroxine-binding globulin (TBG) in a family with partial TBG deficiency in Japanese (TBG-PDJ)" Endocrine Journal. 40. 127-132 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsukasa Noguchi et al.: " Hereditary complete thyroxine-binding globulin deficiency : identification by T3 resin uptake test and DNA analysis" Internal medicine. 32. 6-9 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kyoto Takeda et al.: "Gene screening in Japanese families with thyroxine binding globulin complete deficiency demonstrates that a nucleotide deletion at codon 352 may be a rase specific mutation" Clin Endocrinol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kambe Fukushi et al.: "An additional carbohydrate chain in the variant thyroxine-binding globulin (TBG asp-96) impairs its secretion" Molecular Endocrinology. 6. 443-449 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yuichi Mori et al.: "Thyroxine-binding globulin (TBG) abnormalities and TBG gene.(in Japanese)" Medicina. 31. 302-305 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hisao Seo et al.: "Inherited thyroxine-binding globulin (TBG) abnormalities.(in Japanese)" Clin Endocrinol. 40. 1221-1227 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshitaka Hayashi: "Human thyroxine-binding globulin gene:complete sequence and transcriptional regulation." Molecular Endocrinology. 7. 1049-1060 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Charles H.Emerson: "Gene expression and serum thyroxine-binding globulin are regulated by adrenal status and corticosterone in the rat." Endocrinology. 133. 1192-1196 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ikuo Yamamori: "Gene screening of 23 Japanese families with complete thyroxine-binding globulin deficiency:identification of a nucletide deletion at codon 352 as a common cause." Endocrine Journal. 40. 563-569 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshitaka Miura: "Sequence of a variant thyroxine-binding globulin(TBG)in a family with partial TBG deficiency in Japanese(TBG-PDJ)." Endocrine Journal. 40. 127-132 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tsukasa Noguchi: "Hereditary complete thyroxine-binding globulin deficiency:identification by T3 resin uptake test and DNA analysis." Internal Medicine. 32. 6-9 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kambe Fukushi: "An additional carbohydrate chain in the variant thyroxine-binding globulin(TBG asp-96)impairs its secretion." Molecular Endocrinology. 6. 443-449 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshitaka Miura: "A truncated thyroxine-binding globulin due to a frameshift mutation is retained within the rough endoplasmic reticulum(in press)" The Journal of Clinical Endocrinology & Metabolism.

    • Related Report
      1993 Annual Research Report
  • [Publications] Kyoko Takeda: "Gene screening in Japanese families with thyroxine binding globulin complete deficiency demonstrates that a nucleotide deletion at codon 352 may be a rase specific mutation(in press)" Clinical Endocrinology.

    • Related Report
      1993 Annual Research Report
  • [Publications] 森祐一: "TBG異常症とTBG遺伝子" Medicina. 31. 302-305 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 三浦義孝: "日本人のTBG完全欠損症(TBG-CDJ)の発症機序に関する研究" 名古屋大学環境医学研究所年報. 44. 161-165 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 妹尾久雄: "遺伝性サイロキシン結合グロブリン(TBG)異常症" ホルモンと臨床. 40. 1221-1227 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fukushi Kambe: "An additional carbohydrate chain in the variant thyroxine-binding globulin(TBG asp-96)impairs its secretion" Molecular Endocrinology. 6. 443-449 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yoshitaka Miura,: "Sequence of a variant thyroxine-binding globulin(TBG)in a family with partial TBG deficiency in Japanese(TBG-PDJ)" Endocrine Journal.40. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Noguchi T: "Hereditary complete thyroxine-binding globulin deficiency: identification by T3 resin uptake test and DNA analysis" Internal Medicine. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 三浦 義孝: "日本人のTBG完全欠損症(TBG-CDJ)の発症機序に関する研究" 環研年報,. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 妹尾 久雄,: "遺伝性サイロキシン結合グロブリン(TBG)異常症" ホルモンと臨床. 40. 1221-1227 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 山守 育雄: "TBG完全欠損症のallele specific amplificationを用いた遺伝子スクリーニング" 医学と薬学. 27(5). 1166-1168 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yuichi Mori: "Estrogen increased the accumulation of the thyroxeni-binding globulin(TBG)messenger ribonucleic acid in the rat liver" 66th Meeting American Thyroid association, abstract. S-41 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yoshitaka Miura: "Retention within the rough endoplasmic reticulum may be the cause of complete thyroxine-binding globulin deficiency in Japanese(TBG-CDJ)" 66th Meeting American Thyroid association, abstract. S-37 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yamamori Ikuo: "A nucleotide deletion is universal as a cause of complete thyroxine-binding globulin deficiency in Japanese." The 9th International Congress of Endocrinology,Abstract. 05-01-128 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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