| Project/Area Number |
04671486
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | THE UNIVERSITY OF TOKUSHIMA |
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Principal Investigator |
BANDO Hiroshi The University of Tokushima, School of Medicine Instructor, 医学部・附属病院, 助手 (60228690)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Takehiko The University of Tokushima, School of Medicine Medical staff, 医学部・附属病院, 医員
WAKATSUKI Maki The University of Tokushima, School of Medicine Medical staff, 医学部・附属病院, 医員
SHINTANI Yasumi The University of Tokushima, School of Medicine Instructor, 医学部・附属病院, 助手 (10235773)
石川 雅康 徳島大学, 医学部・附属病院, 医員
岡内 泰弘 徳島大学, 医学部・附属病院, 医員
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Microdialysis / Hypothalamus / Somatostatin / TRH / GHRH / Circadian rhythm / Rat / Microdialysis / Hypothalamus / Somatostatin / Circadian rhythm / Rat / pituitary |
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| Research Abstract |
We inestigated for these two years secretory mechanism of hypothalamic hormones such as somatostatin (SRIF) and Thyrotropin-Releasing hormone (TRH) using method of brain microdialysis, and we now summarize these results.
1) In measuring the extremely small amount of hypothalamic hormones by brain microdialysis method, we clarified the usefulness and adequecy of PC and PS membranes.
2) Secretory mechanism of somatostain (SRIF) from the hypothalamus : We investigated the SRIF concentration in extracellular fluid in rat pituitary gland. The results were follows : The concentration of SRIF in extracellular fluid of the rat pituitary was between 35 to 97 pg/ml, and it showed the circadian rhythm with higher concentration in the light phase (35-55 pg/ml) than in the dark phase (60-97 pg/ml). Furthermore, SRIF concentration occasionally increased in 3-6 hours-interval. When we investigated the influences of various anesthetic agents, SRIF concetration increased with urethane anesthesia, and it decreased with pentobarbital anesthesia.
3) Secretory mechanism of Thyrotropin-Releasing Hormone (TRH) : We investigated the TRH concentration in the paraventricular nucleus in the hypothalamus of the rat. TRH concentration in the dialysate ranged from 7.6 to 10 pg/ml, and it showed higher tendency in the dark phase (2.74 (〕SY.+-.〔) 1/65 pg/ml) than in the light phase (2.15 (〕SY.+-.〔) 1.46 pg/ml).
4) From these results, we clarified the possibility and usefulness of measuring hypothalamic hormones directly and with dinamically by using the method of brain microdialysis.
Furthermore, we are at present investigating the secretory mechanism of Gonadotropin-Releasing Hormone (GnRH) and Growth Hormone-Releasing Hormone (GHRH).
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