| Project/Area Number |
04671488
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
内分泌・代謝学
|
|---|
| Research Institution | KYUSHU UNIVERSITY |
|---|
Principal Investigator |
TAKAYANAGI Ryoichi Kyushu University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (30154917)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
大中 佳三 九州大学, 医学部, 医員
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | Endothelin / Endothelin-converting enzyme / Endothelial cell / Big endothelin / ECE / ET |
|---|
| Research Abstract |
1. Purification and characterization of endothelin-converting enzyme (ECE).
ECE was solubilized with Lubrol PX from membrane fraction of porcine aortic endothelium and was purified by sequential chromatography on DEAE-agarose, Ricinus communis agglutinin 120-agarose, peanut agglutinin-agarose, Mono Q,and TSK3000SW_<XL> columns. Apparent MW was 120 kDa when analyzed on SDS-PAGE under reducing conditions. The C-terminal sequence of His^<27> to Gly^<34> and Trp^<21> at P^1 position of big ET-1 are essential for its substrate activity.
2. cDNA cloning of ECE.
Two types of ECE cDNA,ECE-1alpha and ECE-1beta, were cloned from human umbilical vein endothelial cells. ECE-1beta encodes 758 amino acid residues, which has a single putative transmembrane domain (amino acids 57-77). ECE belongs to NEP and Kell blood-group protein. Further physiological characteristics of ECE has been studied by the expression of these cloned ECE-1cDNAs.
|
