| Project/Area Number |
04680044
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Nagasaki University |
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Principal Investigator |
SATO Hiroshi Nagasaki Univ.Med.Associate Prof., 医学部, 助教授 (50072947)
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| Co-Investigator(Kenkyū-buntansha) |
KISHIKAWA Masao Nagasaki Univ.Med.Associate Prof., 医学部, 助教授 (80112374)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Uirus / Laboratory animal / Pneumonia / Mouse / Rat / Sero-epidemiological method / PVM / HVJ / Penumoria / mouse / Sero-epidenriological method / ウイルス / 実験動物 / 肺炎 / マウス / ラット / 免疫不全 |
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| Research Abstract |
[1] INDIRECT FLUORESCENT ANTIBODY METHOD WAS APPLIED FOR A DETECTION OF PNEUMONIA VIRUS OF MOUSE(PVM)ANTIBODY IN COLONIES OF LABORATOREY ANIMALS IN JAPAN.THE RESULTS SHOWED THAT THE ANTIBODY EXIST IN SPF RATS WITH HIGH INCIDENCE.THE ANTIBODY WAS ALSO DETECTED IN OF RABBITS.HOWEVER,THE ANTIBODY WAS NOT DEMONSTRATED IN THE SERA OF MOUSE.THESE RESULTS SUGGESTED THAT PVM INFECTION IS PRESENT IN LABORATORY RATS IN JAPAN AS WELL AS WESTERN COUNTRIES,AND POINTED OUT THE IMPORTANCE OF MICROBIOLOGICAL MONITORING FOR PVM IN FACILITIES FOR ANIMAL EXPERIMENTATION AND ANIMAL BREEDERS.
[2] A TRIAL OF VIRUS ISOLATION WAS RESULTED IN POSITIVE.THE CHARACTERISTICS OF NEW ISOLATES (31N,14-3)WERE THE SAME AS THE No.15 ; STANDARD-TYPE OF PVM.THE 31N WAS INFECTED TO MICE AND RATS.AS A RESULT,31N INDUCED THE CONSOLIDATION IN LUNG.ALSO,31N INDUCED INTERSTITIAL PNEUMONIA IN MICE AND RATS.THE SEVERITY WAS HEAVIER IN MICE THAN RATS.AS THE RESULTS,THESE HISTO-PATHOLOGICAL FINDINGS IN RATS SUGGESTED THAT RESULTS WERE VERY IMPORTANT TO INVESTIGATOR OF ANIMAL EXPERIMENTATION AND ADMINISTRATOR OF ANIMAL FACILITY.
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