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A study of the topological change of DNA in Escherichia coli induced by heat shock.

Research Project

Project/Area Number 04680153
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

SEKIMIZU Kazuhisa  Kyushu University, Department of Microbiology, Professor, 薬学部, 教授 (90126095)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsDNA topology / DNA supercoiling / DNA topoisomerase / DNA gyrase / heat shock response / heat shock proteins / recombination / Escherichia coli / 熱ショック蛋白 / 相同組み換え
Research Abstract

Heat treatment of wild-type Escherichia coli cells leads to relaxation of negatively supercolied plasmid DNA, followed by a re-supercoiling reaction to the original level of the linking number of DNA.There was no evidence of the recovery of the DNA torsional strain in DNA of gyrA mutant cells. After heat treatment, DnaK and GroEL proteins were synthesized continuously in gyrA mutant cells but only transiently in wild-type cells. Thus, change in superhelical density of the DNA correlates with temperature -induced expression of heat shock proteins.
Inhibitors of DNA gyrase (nalidixic acid, novobiocin), an organic solvent (ethanol) and a psychotropic drug (chlorpromazine) all stimulated relaxation of cellular DNA, over the same concentration range that induced DnaK and GroEL proteins, As DNA relaxation was induced by heat treatment or chemicals in an rpoH, mutant, the process is not the result of any induced synthesis of heat shock proteins.
In rpoH and dnaK mutants, a re-supercoiling reaction did not occur, hence DnaK protein and other heat shock proteins presumably participate in the reaction. We propose that DNA relaxation induced by heat treatment stimulates synthesis of heat shock proteins involved in the re-supercoiling of DNA, which in turn shuts off the induction of these proteins.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] T.Mizushima,S.Natori and K.Sekimizu.: "Relaxation of supercoiled DNA associated with induction of heat shock proteins in Escherichia coli." Mol.Gen.Genet.238. 1-5 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Haq,S.Natori and K.Sekimizu.: "Heat-induced DNA relaxation in vitro by mouse DNA topoisomerase I in" J.Biochem.113. 620-624 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Ogata,T.Miki and K.Sekimizu.: "A role of heat shock proteins for homologous recombination in Escherichia coli." Biochem.Biophys.Res.Commun.197. 34-39 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Ogata,T.Mizushima,K.Kataoka,T.Miki and K.Sekimizu.: "Identification of DNA topoisomerases in volved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli." Mol.Gen.Genet.(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Mizushima, S.Natori and K.Sekimizu: "Relaxation of supercoiled DNA associated with induction of heat shock proteins in Escherichia coli." Mol.Gen .Genet.238. 1-5 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Hag, S.Natori and K.Sekimuzu: "Heat-induced DNA relaxation in vitro by mouse DNA topoisomerase I in the presence of ethidium bromide." J.Biochem.113. 620-624 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Ogata, T.Miki and K.Sekimizu: "A role of heat shock proteins for homologous recombination in Escherichia coli." Biochem.Biophys.Res.Commun.197. 34-39 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Ogata, T.Mizushima, K.Kataoka, T.Miki and K.Sekimizu: "Identification of DNA topoisomerases involved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli." Mol.Gen.Genet. in press. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sagara,Wada,Takata,Waterman,Sekimizu,Horiuchi: "Direct Expression of Adrenodoxin Reductase in Escherichia coli and the Functional Characterization" Biol.Pharm.Bull.16. 627-630 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mizushima,Natori,Sekimizu: "Relaxation of supercoiled DNA associated with induction of heat shock proteins in Escherichia coli" Mol Gen Genet. 238. 1-5 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hirai.Takeda.Natori.Sekemizu: "Inhibition of SV40 DNA Replication in Vitro by Chlorpromazine" Biol.Pharm.Bull.16. 565-567 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Haq.Natori,Sekimizu: "Heat-Induced DNA Relaxation In Vitro by Mouse DNA Topoisomerase I in the Presence of Ethidium Bromide" J.Biochem.113. 620-624 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takata.Sagara.Kono.Sekimizu.Horiuchi,: "Gene Structure of Borine Adrenodoxin Reductase" Biol.Pharm.Bull.16. 1200-1206 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mizushima,Natori.Sekimizu: "Inhibition of DNA Synthesis in Meth A Cells by Chlorpromazine" Biol.Pharm.Bull.16. 953-955 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sagara,Wada,Takata,Waterman,Sekimizu,Horiuchi: "Direct Expression of Adrenodoxin Reductase in Escherichia coli and the Functional Characterization" Biol.Pharm.Bull.16. 627-630 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mizushima,Naori,Sekimizu: "Relaxation of supercoiled DNA associated with induction of heat shock proteins in Escherichia coli" Mol Gen Genet. 238. 1-5 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hirai.Takeda.Natori.Sekimizu: "Inhibition of SV40 DNA Replication in Vitro by Chlorpromazine" Biol.Pharm.Bull.16. 565-567 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Haq.Natori,Sekimizu: "Heat-Induced DNA Relaxation In Vitro by Mouse DNA Topoisomerase I in the Presence of Ethidium Bromide" J.Biochem.113. 620-624 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takata,Sagara.Kono.Sekimizu.Horiuchi,: "Gene Structure of Borine Adrenodoxin Reductase" Biol.Pharm.Bull.16. 1200-1206 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mizushima,Natori,Sekimizu: "Inhibition of DNA Synthesis in Meth A Cells by Chlorpromazine" Biol.Pharm.Bull.16. 953-955 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tomura,Ishikawa,Sagara.Miki,Sekimizu: "Requiremeut of phosphatidylglycerol for flagellation of Escherichia coli" FEBS LETTERS. 329. 287-290 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ogata,Miki,Sekimizu: "A Role of Heat Shock Proteins for Homologous Recombination in Escherichia coli" BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 197. 34-39 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Koga,Sagara,Yaoi,Tsujimura Nakamura,Sekimizu,Makino Shimada,Ishimura,Yura,Go,Ikeguchi,Horiuchi.: "Essential role of the Arg^<112> residue of cytochrome P450 cam for electron transfer from reduced putidaredoxin" FEBS LETTERS. 331. 109-113 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takeda,Sagara,Kita,Natori,Sekimizu: "Purification of a GTP-Binding Protein Localized in Mitochondria" J.Biochem. 114. 684-690 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ogata,Mizushima,Kataoka,Miki,Sekimizu: "Identification of DNA topoisomerases in volved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli" Mol,Gen,Genet,. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tomura,Ishikawa,Sagara,Miki,Sekimizu: "Requiremeut of phosphatidylglycerol for flagellation of Escherichia coli" FEBS LETTERS. 329. 287-290 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ogata,Miki,Sekimizu: "A Role of Heat Shock Proteins for Homologous Recombination in Escherichia coli" BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 197. 34-39 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Koga,Sagara,Yaoi,Tsujimura,Nakamura,Sekimizu,Makino,Shimada,Ishimura,Yura,Go,Ikeguchi,Horiuchi: "Essential role of the Arg^<112> residue of cytochrome P450 cam for electron transfer from reduced putidaredoxin" FEBS LETTERS. 331. 109-113 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takeda,Sagara,Kita,Natori,Sekimizu: "Purification of a GTP-Binding Protein Localized in Mitochondria" J.Biochem. 114. 684-690 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ogata,Mizushima,Kataoka,Miki,Sekimizu: "Identification of DNA topoisomerases in volved in immediate and transient DNA relaxation induced by heat shock in Escherichia coli" Mol,Gen,Genet.(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hag,Natori,Sekimizu: "Heat-induced DNA relaxation in vitro by movie DNA topoisomevase I in the presence of ethidium bromide" J.Biochem. 113. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sagara,Wada,Takata,Waterman,Sekimizu,Horiuchi: "Direct Expression of Adrenodoxin Reductase in Escherichia coli and the Functional Characterigation" Biol.Phavm.Bull. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirai,Takeda,Natori,Sekimizu: "Inhibition of SV40 DNA Replication In Vitro by Chlorpromazine" Biol.Pharm.Bull.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Mizushima,Natori,Sekimizu: "Relaxation of supetoiled DNA associated with induction of heat shock proteins in Escherichia cob" Mol.Gen.Genet.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tanji,Ohta,Kawato,Mizushima,Natori,Sekimizu: "Decrease by psychotropic drugs and local anesthetics of membrare fluidity measured by fluorescence anisotropy in Escherichia coli" J.Pharm.Pharmacol. 44. 1036-1037 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Mizushima,Natori,Sekimizu: "Inhibition of Escherichia coli DNA topoisomerase I activity by phospholipids" Biochem.J.285. 503-506 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nakanishi,Nakano,Nomura,Sekimizu,Natori: "Puritication,Gene Cloning,and Gene Disruption of the Transcription Elongation S-II in Saccharomyces cerevisiae" J.Biol.Chem.267. 13200-13204 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirai,Natori,Sekimizu: "Reversal by Phosphatidylglycerol and Cardiolipin of Inhibition of Transcription and Replication by Histones in Vitro" Arch.Biochem.Biophys.298. 458-463 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tanji,Mizushima,Natori,Sekimizu: "Induction by psychotropic drugs and local anesthetics of DnaK and GroEL proteins in Escherichia coli" Biochim,Biophys,Acta. 1129. 172-176 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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