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Amino-terminal processing of intracellular proteins and its biological functions

Research Project

Project/Area Number 04680162
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionOsaka University

Principal Investigator

NORIOKA Shigemi (1993)  Osaka Univ., Institute for Protein Research, Assistant Professor, 蛋白質研究所, 助手 (70198638)

綱澤 進 (1992)  大阪大学, たんぱく質研究所, 助教授 (30029962)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsMethionine Aminopeptidase / N-acetyltransferase / N-terminal processing / Acetylation / Initial Methionine / N-terminal rule / Ubiquitin / Proteolysis / アセチルトランスフェラーゼ / プロセシング / アシルアミノ酸遊離酵素 / アミノアシラーゼ / イニシェーター〓チオニン / 小細胞性肺がん
Research Abstract

Amino-terminal processing of proteins is a very common event. In eukaryotes, translation of proteins is initiated with methionine (Met^i), and more than half of the methionine residue jis cleaved. Subsequently, 60-80% of the intracellular proteins are amino-terminally blocked by acetylation. In this project, I have tried to elucidate the enzyme(s) system related to such amino-terminal processing and further to estimate its biological functions. As the first trial, I have found at least three enzymes are engaged in the event in yeast, Saccharomyces cerevisiae. One is a methionine aminopeptidase (MAP), which specifically removes Met^i from certain proteins before the completion of nascent polypeptide chains, and the others are two N-acetyltransfeases with diffrent specificity, Nat1 and Nat2. Nat1 acts on acetylation for proteins having glycine, alanine, serine, threonine, proline and valine, whereas Nat2 acts only on amino-terminal acetylation for Met-Asp/-Glu/-Asn sequence. Further studies have clarified some molecular properties for each enzymes. For the biological functions of amino-terminal processing, it is suggested that the event tends to convert amino-terminal residues to stabilizing residues in N-end rule presented on the ubiquitin dependent protein degradation sysytem in cell.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Masanori Mitta et al.: "The primary structure of porcine aminoacylase I deduced from cDNA sequence." J.Biochem.112. 737-742 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masaru Miyagi et al.: "Determination of the covalent structure of aminoacylase from porcine kidney by ion-spray ionization mass spectrometry." J.Protein Chem.11. 378-379 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Susumu Tsunasawa et al.: "Does uncontrolled survial of acetylated peptides lead to cell?" J.Laboratory and Clinical Medicine. 120. 505-506 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fred Sherman et al.: "Amino-terminal acetylation of altered form of yeast iso-1-cytochromes c in momal and nat 1^- strains of yeast." J.Protein Chem.11. 380-382 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hikaru Takakura et al.: "NH_2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." J.Biol.Chem.267. 5442-5445 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masanori Mitta et al.: "The nucleotide sequence of human aminoacylase I." Biochim.Biophys.Acta. 1174(2). 201-203 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Susumu Tsunasawa: "The post-translational modification of proteins." Japan Scientific Societ Press, 281 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 綱沢 進: "Methods in Protein Sequence Analysis" 今堀和友,崎山文夫, 310 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masanori MItta et al.: "The primary structure of porcine aminoacylase I deduced from cDNA sequence." J.Biochem.112. 737-742 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masaru Miyagi et al.: "Determination of the covalent structure of amino-acylase from pocine kidney by ion-spray ionization mass spectrometry." J.Protein Chem.11. 378-379 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Susumu Tsunasawa et al.: "Does uncontrolled survial of acetylated peptides lead to cell ?" J.Laboratory and Clinical Medicine. 120. 505-506 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fred Sherman et al.: "Amino-terminal acetylation of altered form of yeast iso-1-cytochromes c in normal and nat^- strains of of yeast." J.Protein Chem.11. 380-382 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hikaru Takakura et al.: "NH_2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." J.Biol. Chem.267. 5442-5445 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masanori Mitta et al.: "The nucleotide sequence of human aminoacylase 1." Biochim. Biophys. Acta. 1174(2). 201-203 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Susumu Tsunasawa: The post-translational modification of proteins.Japan Scientific Societ. Press, 281 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Susumu Tsunasawa: Deblocking and subsequent microsequence analysis of N-terminally blocked proteins immobilized on PVDF membrance.Plenum, 310 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mitta M.: "The nucleotide sequence of humon aminoacylase-1." Biochim.Biophys.Acta. 1174(2). 201-3 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 綱沢 進: "Methods in Protein Sequence Analysis" 今堀和友、崎山文夫, 310 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mitta,Masamori: "The primary structure of porcine aminoacylase 1 deduced from cDNA sequence" Journal of Biochemistry. 112. 737-742 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Miyagi,Masaru: "Determination of the covalent structure of aminoacylase from porcine kidney by ion-spray ionization mass spectrometry" Journal of Protein Chemistry. 11. 378-379 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tsunasawa,Susumu: "Does uncontrolled survial of acetylated peptides lead to cell psoliferation?" Journal of Laboratory and Clinical Medicine. 120. 505-506 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fred Sherman: "Amino-terminal acetylation of altered form of yeast iso-1-cytochromes c in normal and natl-strains of yeast" Journal of Protein Chemistry. 11. 380-382 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takakura,Hikaru: "NH_2-terminal acetylation of ribosamal proteins of Sacoharomyces cereviside" Journal of Biological Chemistry. 267. 5442-5445 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tsunaswa,Susumu.: "The post-translational modification of proteins" Japan Scientific Societ,Press, 281 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 綱沢 進: "食品の生体調節機能の解析" 学会出版センター, (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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