| Project/Area Number |
04680174
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | NIHON UNIVERSITY |
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Principal Investigator |
ANZAI Kaijiro NIHON UNIVERSITY, COLLEGE OF PHARMACY, ASSOCIATE PROFESSOR, 薬学部, 助教授 (30114359)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Repetitive Sequence / Low Molecular Mass RNA / Neuron / Dendritic RNA / Protein Synthesis / RNA Transport / Ribonucleoprotein Particle / DNA- and RNA-Binding Protein / 低分子量 RNA / RNA / リボ核タンパク粒子 / DNA結合タンパク / RNA結合タンパク / 樹状突起 |
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| Research Abstract |
BC-1 RNA is a brain-specific small RNA transcript of identifier sequences present in the somas and dendrites of neurons. We recently reported that the RNA is complexed with a protein(s) to form a 10S ribonucleoprotein particle. We demonstrate that this10S BC-1 ribonucleoprotein particle contains a DNA-binding protein(s)(Bp-1 protein) capable of interacting with a region between split promoter sequences for RNA polymerase III within the identifier sequences. The region has short inverted repeats : aperfect octanucleotide repeat and an imperfect heptanucleotide repeat, each of which contains a GCAAG/CTTGC motif. We also demonstrate that the binding of this protein either to the array of pentamer motifs or to BC-1 RNA is mutually exclusive. The mol-ecular masses of photocross-linking adducts of Bp-1 protein to a 32P-labeled GCAAG/CTTGC motif-specific probe were estimated to be about 31 and 36 kDa, indicating that two species of Bp-1 proteins may be present in the brain.
We also demonstrate an accumulation of Bp-1 protein in the nucleus of brain cells from mouse fetus and newborns that precedes the postnatal increase in BC-1 RNA.Cyt-oplasmic Bp-1 protein present in a complex with BC-1 RNA increases postnatally with a concomitant decrease in nuclear Bp-1 protein. These observations suggest that Bp-1 protein may play a role(s) in the synthesis and nuclear export of BC-1 RNA.
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