| Project/Area Number |
04680189
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
代謝生物化学
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| Research Institution | Osaka University |
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Principal Investigator |
NIHIRA Takuya Osaka University Dept. Biotechnology Associate Professor, 工学部・応用生物工学科, 助教授 (70144441)
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| Co-Investigator(Kenkyū-buntansha) |
SAKUDA Shohei Osaka University Dept. Biotechnology Research Associate, 工学部・応用生物工学科, 助手 (80192087)
YAMADA Yasuhiro Osaka University Dept. Biotechnology Professor, 工学部・応用生物工学科, 教授 (00011891)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Streptomyces / vbrA / secondary metabolism |
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| Research Abstract |
The presence of the vbrA gene encoding the transcriptional antiterminator NusG equvalent protein of Streptomyces virginiae was tested for in 73 Soteptomyces species by Southern hybridization. Fifty-five strains (75%) including S.griseus, S.lividans TK-21 and S.coelicolor A3(2) showed clear hybridization signals, indicating wide distribution of vbrA or vbrA homologs in Streptomyces species. With hybridization patterns against 3 different probes, i.e., probes covering vbrA alone, the downstream gene rplK alone, and both vbrA-rplK, the 55 strains were classified into 4 groups. In the group I, II and III (total 50 strains) vbrA was found to be adjcent to rplK, indicating that the gene arrangement vbrA-rplK is common in Streptomyces and that these gene may constitute a part of gene cluster encoding several components of the transcriptional and translational apparatus, as in Escherichia coli.
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