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Observation of wet biological specimens by imaging x-ray microscope with the use of zone plates

Research Project

Project/Area Number 04680277
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionKansai Medical University (1993)
Jichi Medical School, School of Nursing (1992)

Principal Investigator

KIHARA Hiroshi  Kansai Medical University, Dept.of Medicine, Professor, 医学部, 教授 (20049076)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsimaging x-ray microscope / zone plate / synchrotron radiation / UVSOR / X線顕微鏡
Research Abstract

We have mounted imaging x-ray microscope with two zone plates at UVSOR(Okazaki, Japan), and applied it to observe various bio-specimens dry or wet. With a zone plate as a test specimen, we observed zones as fine as 64 nm, which was very close to theoretical resolution limit (55nm).
As dry specimens, we observed specimens such as diatom, myofibril, clamidomonas, of which pictures are shown in the main volume of the present activity report. As wet specimen, we only observed an image of myofibril or its fragment, as the limitation of usable beam time.
we realized that we still have several probelems to be solved in future, mainly concerning the condenser zone plate ; specimens are not illuminated uniformly, probably due to the mismatching of optical parameters of condenser zone plate to the micro zone plate, or due to the deformation of the consenser zone plate from the designed values.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plates II" UVSOR activity report. 1991. 72-73 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Observation of Wet Bio-Specimen by X-Ray Imaging Microscope with ZonePlate at UVSOR" Jpn.J.Appl.Phys.31. L1571-L1573 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plates at UVSOR" Proc.SPIE. 1741. 85-93 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Sub-Optical Resolution at UVSOR" UVSOR activity report. 1992. 137-138 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plates at UVSOR I:Its Performance" Jpn.J.Appl.Phys.32. 4791-4798 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plates at UVSOR" UVSOR activity report. 1993. 163-164 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Fujisaki et al.: "X-Ray Microscopy III(X-Ray Focusing and Imaging Properties of a Nickel Phase Zone Plate,pp.90-93)" Springer-Verlag,Berlin, 491 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "X-Ray Microscopy III(Soft X-Ray Microscopy with Zone Plates at UVSOR,pp.147-150)" Springer-Verlag,Berlin, 491 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plates II" UVSOR activity report. 1991. 72-73 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Observation of Wet Bio-Specimen by X-Ray Imaging Microscope with Zone Plate at UVSOR" Jpn.J.Appl.Phys.31. L1571-L1573 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plates at UVSOR" Proc.SPIE. 1741. 85-93 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Sub-Optical Resolution at UVSOR" UVSOR activity report. 1992. 137-138 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plate at UVSOR I : Its Performance" Jpn.J.Appl.Phys.32. 4791-4798 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plates at UVSOR" UVSOR activity report. 1993. 163-164 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Fujisaki et al.: X-Ray Focusing and Imaging Properties of a Nickel Phase Zone Plate. in "X-Ray Microscopy III(R.E.Burge et al., ed.)", Springer-Verlag, Berlin, 90-93(491) (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: Soft X-Ray Microscopy with Zone Plates at UVSOR. in "X-Ray Microscopy III(R.E.Burge et al., ed.)", Springer-Verlag, Berlin, 147-150(491) (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Watanabe et al.: "Soft X‐Ray Microscope with Zone Plates II" UVSOR activity report. 1991. 72-73 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Watanabe et al.: "Observation of Wet Bio‐Specimen by X‐Ray Imaging Microscope with Zone Plate at UVSOR" Jpn.J.Appl.Phys.31. L1571-L1573 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Watanabe et al.: "Soft X‐Ray Microscope with Zone Plates at UVSOR" Proc.SPIE. 1741. 85-93 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Watanabe et al.: "Soft X‐Ray Microscope with Sub‐Optical Resolution at UVSOR" UVSOR activity report. 1992. 137-138 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Watanabe et al.: "Soft X‐Ray Microscope with Zone Plates at UVSOR I: Its Performance" Jpn.J.Appl.Phys.32. 4791-4798 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Watanabe et al.: "Soft X‐Ray Microscope with Zone Plates at UVSOR" UVSOR activity report. 1993(in pr). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Fujisaki et al.: "X‐Ray Microscopy III" X‐Ray Focusing and Imaging Properties of a Nickel Phase Zone Plate, 491(90-93) (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Watanabe et al.: "X‐Ray Microscopy III" Soft X‐Ray Microscopy with Zone Plates at UVSOR, 491(147-150) (1992)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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