| Project/Area Number |
04806007
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
NAMBA Shigetou THE UNIVERSITY OF TOKYO,DEPARTMENT OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (50189221)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | custom PCR / genus Potyvirus / coat protein mediated protection / Nicotiana benthamiana / トランスジェニック植物 / 外被タンパク質 / ウイルス病抵抗性 / キュウリモザイクウイルス / パパイヤリングスポットウイルス / ズッキーニイエロ-モザイクウイルス / カボチャモザイクウイルスII / Potyvirus / 組換え植物 / 形質転換 / 植物ウイルス / ウイルス外被タンパク質 / 抵抗性 / potyvirus / ELISA / アグロバクテリウム |
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| Research Abstract |
Coat protein (CP) genes ofseveral isolates of CMV and many species of genus Potyvieus were engineered and transferred into tobacco and Nicotiana benthamiana by custom PCR (CPCR) engineering. Transformed plants gave high livels of CP expression and exhibited broad form coat protein mediated resistance to potyviruses. This suggests the feasibility of using a limited number of potyviruses to get protection against a broad range of potyviruses.
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