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RESEARCH FOR EXPRESSION MECHANISMS OF BROAD SPECTRUM PESISTANCE OF TRANSGENIC PLATS AGAINST VIRUS DESEASES

Research Project

Project/Area Number 04806007
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

NAMBA Shigetou  THE UNIVERSITY OF TOKYO,DEPARTMENT OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (50189221)

Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordscustom PCR / genus Potyvirus / coat protein mediated protection / Nicotiana benthamiana / トランスジェニック植物 / 外被タンパク質 / ウイルス病抵抗性 / キュウリモザイクウイルス / パパイヤリングスポットウイルス / ズッキーニイエロ-モザイクウイルス / カボチャモザイクウイルスII / Potyvirus / 組換え植物 / 形質転換 / 植物ウイルス / ウイルス外被タンパク質 / 抵抗性 / potyvirus / ELISA / アグロバクテリウム
Research Abstract

Coat protein (CP) genes ofseveral isolates of CMV and many species of genus Potyvieus were engineered and transferred into tobacco and Nicotiana benthamiana by custom PCR (CPCR) engineering. Transformed plants gave high livels of CP expression and exhibited broad form coat protein mediated resistance to potyviruses. This suggests the feasibility of using a limited number of potyviruses to get protection against a broad range of potyviruses.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Candresse,T.: "Family description:Trichovirus.In classification and nomenculture of viruses,virus report." Archives of viralogy. (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Namba,S.: "Familly description:Capillovirus.On classification and nomenculture of viruses with report." Archives of Virology. (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Martelli,G.P.: "Trichovirus,a new genus of plant viruses" Archives of Virology. 134. 451-455 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Gonsalves,C.: "Transfer of cucumber mosaic virus-white leaf strain coat protein gene into Cucumis melo by A.tumefaciens and microprojectiles and evaltuation of tranagenic plants for protection against CMV infections." J.Amer.Soc.Hort.Sci.119(2). 345-355 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohira,K.: "Nucleotide sequence of the 3'-terminal region of citrus tatter leaf virus RNA." Virus Genes. 8. 169-172 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohira,K.: "Nucleotide sequence of the coat protein coding region of tulip breaking virus." Viirus Genes. 8. 165-167 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hidaka,M.: "Cloning and sequencing of the 3' half of a potato virus Y (0 strain) genome encoding the 5k protein protease,polymerase and coat protein." Nuc.Acids Res.20. 3515 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Namba,S.: "Protecition of transgenic plants expressing the coat protein geme of watermelon mosaic virus II or zucchini yellow mosaic against six potyviruses." Phytopatholgy. 82(9). 940-946 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ochi,M.: "Nucleotide sequence of the 3'-terminal region of potato virus tRNA." Ann.Phytopath.Soc.Japan. 58(3). 416-425 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Candresse, T.: "Family description : Trichovirus. In Classification and Nomenclature of Viruses, VIth Report." Archives of Virology (Supplementum). (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Namba, S.: "Family description : Capillovirus. In Classification and Nomenclature of Viruses, VIth Report." Archives of Virology (Supplementum). (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Martelli, G.P.: "Trichovirus, a new genus of plant viruses" Archives of Virology. 134. 451-455 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Gonsalves, C.: "Transfer of cucumber mosaic virus-white leaf strain coat protein gene into Cucumis melo by A.tumefaciens and microprojectiles and evaluation of transgenic plants for protection against CMV infections." J.Amer.Soc.Hort.Sci.119 (2). 345-355 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohira, K.: "Nucleotide sequence of the 3'-terminal region of citrus tatter leaf virus RNA." Virus Genes. 8. 169-172 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohira, K.: "Nucleotide sequence of the coat protein coding region of tulip breaking virus." Virus Genes. 8. 165-167 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hidaka, M.: "Cloning and sequencing of the 3' half of a potato virus Y (O strain) genome encoding the 5k protein, protease, polymerase and coat protein." Nuc.Acids Res.20. 3515 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Namba, S.: "Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II or zucchini yellow mosaic virus against six potyviruses." Phytopathology. 82 (9). 940-946 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ochi, M.: "Nucleotide sequence of the 3'-terminal region of potato virus T RNA." Ann.Phytopath.Soc.Japan. 58 (3). 416-425

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Namba et al.: "Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II or zucchini yellow mosaic virus against six potyviruses." Phytopathology. 82. 940-946 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Namba et al.: "Transfer of cucumber mosaic virus-white leaf strain coat protein gene into Cucumis melo by A.tumefaciens and microprojectiles and evaluation of transgenic plants for protection against CMV infections." J.Amer.Soc.Hort.Sci.119. 345-355 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 難波成任: "Potyvirus抵抗性の分子育種" 植物細胞工学. 5. 101-109 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] Namba,S.: "Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II and zucchini yellow mosaic virus against potyvirus." Phytopathology. 82. 940-946 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ochi,M.: "Nucleotide sequence of the 3'-terminal region of potato virus T RNA." 日植病報. 58. 416-425 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidaka,M.: "Cloning and sequencing of the 3' half of a potato virus Y(Ostran)geneme encoding the 5K protein,protease,polymerase and coat protein." Nuc.Acids Res.20. 3515 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohira,K.: "Nucleotide sequence of the coat protein coding region of TBV." Virus Genes. 8. 165-167 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Namba,S.: "Family description:Capillovirus.In Classification and Nomenclature of Viruses,VIth ICTV Report." Archives of Virology(Supplementum). (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Gonsalves,C.: "Transfer of CMV-WL coat protein gene into Cucumis melo by A.tumefaciens and microprojectiles and evaluation of transgenic plants..." Horticultural Science. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Namba,S.ら: "Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II or zucchini yellow mosaic virus against six potyviruses." Phytopathology. 82. 940-946 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ochi,M.ら: "Nucleotide sequence of the 3'‐terminal region of potato virus T RNA." Ann.Phytopath.Soc.Japan. 58. 416-425 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Morita,Y.ら: "A new disease of Phalaenopsis and Doritaenopsis caused by Nectria haematococca." Aun.Phytopath.Soc.Japan. 58. 452-455 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hidaka,M.ら: "Cloning and sequencing of the 3'half of a patato virus Y(Ostrain)genome encoding the 5K protein,protease,polymerase and coat protein." Nuc.Acids.Res.20. 3515- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 難波 成任: "potyvirus抵抗性の分子育種" 植物細胞工学. 4. 101-109 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 難波 成任: "16S rRNA遺伝子のPCR増幅による植物病原MLOの検出と分類" 植物防疫. 47. 38-45 (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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