• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Immunoproliferative Microenvironment in the Spleen-An Immunohistological Study.

Research Project

Project/Area Number 04807002
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKUMAMOTO UNIVERSITY

Principal Investigator

MATSUNO Kenjiro  Kumamoto Univ.School of Medicine, Lecturer, 医学部, 講師 (20094047)

Co-Investigator(Kenkyū-buntansha) EZAKI Taichi  Kumamoto Univ.Medicine, Research Assistant, 医学部, 助手 (10128259)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsImmunohistology / Polychromatic Immunostaining / Spleen / Antibody Forming Cell / Macrophage / Cellular Interactions / Microenvironment / Rosette Formation
Research Abstract

(Aim of this study) We examined immunohistologically the cellular rosettes formed by ED2-positive macrophages and antibody forming cells in the rat spleen. The aim of this study is to know the correlation between various types of immune response and the rosette formation and to consider the functional role of the rosette as an immunoproliferative microenvironment.
(Results)(1992)(1) We demonstrated that ED2-positive macrophages also form rosettes with erythroblasts in the red pulp cord (Published just before getting this grant). (2) We examined isotypes of anti-FITC antibody forming cells and found that they bore mainly an IgM isotype (unpublished data). (3) The immunoelectron microscopical analysis is now undergoing.(1993)(1) We prepared an antigen, FITC-hemocyanin, and found that antibody forming cells to this antigen also formed rosettes with ED2-positive macrophages in the outer PALS (unpublished data). We also studied Plasmodium-infected rat spleens. We found that antibody forming … More cells and ED2-positive macrophages considerably increased in number and formed rosettes together in the PALS (presented at an international congress, in preparation). (2) This project stands on a still preliminary field of research, "Immunohistology", which needs further study to be established. However, we propose this kind of approach as a new and very important one. In order to demonstrate some crops of this field, we have produced and studied a rat experimental pancreatitis model. We found a massive increase of macrophages in the inflamed tissues (Gastroenterology, 1993) and demonstrated a proliferation of macrophage subpopulations by the double immunostaining technique (Arch.Histol.Cytol., 1993).
(DISCUSSION AND FUTURE PROJECT) We consider that the rosette formation of ED2-positive macrophages and antibody forming cells is a common phenomenon producing the microenvironment for the antibody forming cell response not only to various types of artificial antigen but also to infectious microorganisms. Furthermore, they may slso constitute the microenvironment for hemopoietic cell lineages. We plan to immunostain the rosettes with double color fluorescent probes and examine the three dimensional structure of them with the aid of a confocal laser scanning microscope. Less

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Yasuo Yamaguchi: "In situ Kinetics of Acinar,and Inflammatory Cells in Duct Ligation-Induced Pancreatitis in Rats" GASTROENTERLOGY. 104. 1498-1506 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mataro Goto: "Proliferation Kinetics of Macrophage Subpopulations in a Rat Experimental Pancreatitis Model" ARCH.HISTOL.CYTOL.56. 75-82 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Yamaguchi, K.Matsuno, M.Goto, and M.Ogawa: "In Situ Kinetics of Acinar, Duct and Inflammatory Cells in Duct Ligation Pancreatitis in Rats." Gastroenterology. 104. 1498-1506 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Goto, K.Matsuno, Y.Yamaguchi, T.Ezaki, and M.Ogawa: "Proliferation Kinetics of Macrophage Subpopulations in a Rat Experimental Pancreatitis Model." Arch.Histol.Cytol.56-1. 75-82 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasuo Yamaguchi: "In situ Kinetics of Acinar,and Inflammatory Cells in Duct Ligation Induced Pancreatitis in Rats" GASTROENTERLOGY. 104. 1498-1506 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mataro Goto: "Proliferation Kinetics of Macrophage Subpopulations in a Rat Experimental Pancreatitis Model" ARCH.HISTOL.CYTOL.56. 75-82 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松野 健二郎: "脾臓の液性免疫応答の場" 細胞. 24. 110-115 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasuo Yamaguchi: "In Situ Kinetics of Acinar,Duct and Inflammatory Cells in Duct Ligation Pacreatitis in Rats" GASTRO ENTEROLOGY. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Mataro GOTO: "Proliferation Kinetics of Macrophage Subpopulations in a Rat Experimental Pancreatitis Model" ARCH.HISTOL.CYTOL.56. 1-8 (1993)

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi