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Immunohistochemical and molecular biological analysis of neuronal migration

Research Project

Project/Area Number 04807005
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionGunma University (1993)
Keio University (1992)

Principal Investigator

SHIRAO Tomoki  Gunma Univ. Sch. Med. Dept. Neurobiol & Behave. Professor, 医学部, 教授 (20171043)

Co-Investigator(Kenkyū-buntansha) HAYASHI Kensuke  Gunma Univ. Sch. Med. Dept. Neurobiol & Behave. Assistant Professor, 医学部, 助手 (50218567)
KOBAYASHI Satoshi  Keio Univ, Sch. Med. Dept. Physiol. Instructor, 医学部, 助手
三浦 正幸  慶應義塾大学, 医学部, 助手 (50202338)
阿相 晧晃  慶應義塾大学, 医学部, 講師 (30104160)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Keywordsdrebrin / neural development / cell migration / cytoskeleton / actin / actin-binding-protein / ***** / 可塑性 / 成熟脳 / N-CAM / 嗅球 / ラット / ラディアルグリア
Research Abstract

Drebrins are developmentally regulated brain proteins which were first isolated from chick embryonic brain. Immunochemical analysis has revealed that they are concentrated in submembraneous regions of neurons, and the embryonic form of drebrin is pouportedly concerned with neuronal migration during development. In this study we immunohistochemically analyzed the expression of drebrin, embryonic N-CAM, SVP38, GFAP and vementin within the migrating subependymal cells, which are considered to be the postmitotic precursors of granule cells in the rat olfactory bulb, or to be glial progenitor cells. Some of the cells, which attached to one another, expressed drebrin and embryonic N-CAM.These immunoreactive cells made a stream from the anterior horn of lateral ventrice to the olfactory bulb. Since glial cells do not express drebrin, these immunoreactive cells are thought to be migrating neuroblasts.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] H.Asada,K.Uyemura & T.Shirao: "Actin-binding protein,drebrin,accumudates in submembranous regions in parallel with neuronal diff〓" J.Neuroscience Research. (予定). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Toda,T.Shirao,S.Minoshima,N.Shimizu,S.Toya,K.Uyemura: "Molecular cloning of cDNA encoding the actin-binding protein,human drebrin E and chromosonal mapping of drebrin gene #."" Biochem.Biophysi.Res.Com.196. 468-472 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.K.Inoue,T.Shirao: "Process of neurite formation and genetic engineering." J.Neural Transplantation and Plasticity. 3. 287 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Kojima,T.Shirao,K.Obata: "Molecular cloning of a developmentally regulated brain protein chicken drebrin A and its expression by alternative splicing of the drebrin gene." Mol.Brain Res. 19. 101-114 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 白尾智明,石川良樹,林謙介,小林聡: "ラットドレブリンのアクチン結合部位の解析" 神経化学. 32. 236-237 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 池田圭朗,白尾智明,戸谷重雄,植村慶一: "非神経細胞・発現したドレブリン蛋白が細胞接着に及ぼす影響" 神経化学. 32. 358-359 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] "Molecular coning of a developmentally regulated brain protein chicken drebrin A and its expression by alternative splicing of the drebrin gene" Mol. Brain Res. 19. 101-114

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] "Process of neurite formation and genetic engeneering" J.Neural Transplantation and Plasticity. 3. 287

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] "Moleculer cloning of cDNA encoding the actin-binding-protein, human drebrin E and chromosomal mapping of drebrin gene" Biochem. Biophyst. Res. Com. 196. 468-472

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Asada,K.Uyemura & T.Shiruo: "Actin-binding protein,drebrin,accumulates in sabmembranous regions in parallel with nearonal differentiation" J.Newoscienee Research. (予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Toda,T.Shirao,S.Minoshima,N.Shimizu,S.Toya,K.Uyemura: "Molecular cloniny of cDNA encoding the actin-binding protein,human drebrin E and chromosonal mapping of drebrin gene #." Biochem.Biophysi,Res.Com.196. 468-472 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.K.Inoue,T.Shirao: "Process of neurite formation and genetic engineering" J.Neural Transplantation and Plasticity. 3. 287 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Kojima,T.Shirao,K.Obata: "Molecalar cloning of a developmentally regulated brain protein chicken drebrin A and its expression by alternative splicing of the drebrin gene" Mol.Brain Res. 19. 101-114 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 白尾智明,石川良樹,林謙介,小林聡: "ラットドレブリンのアクチン総合部位の解析" 神経化学. 32. 236-237 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 池田圭朗,白尾智明,戸谷重雄,植村〓一: "非神経細胞・発現したドレブリン蛋白が細胞接着に及ぼす影響" 神経化学. 32. 358-359 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 白尾 智明: "Cloning of drebrin A and induction of neurite-like processes in drebrin-transfected cells." Neuro Report. 3. 109-112 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 今村 一之: "Changes of drebrin expression in the visual cortex of the cat during development." Neurosci. Res.13. 33-41 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Peter Kaub: "Identification of rat drebrin embryonic isoform by polymerase chain reaction and its genomic analysis." Bulletin Jap. Neurochem. Soc.31. 148-149 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 白尾 智明: "神経発生における神経細胞骨格関連タンパク質の役割" 日本薬理学雑誌. 100. 465-473 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2017-03-24  

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