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Ribosomal protein gene expression and its regulation by oncoproteins

Research Project

Project/Area Number 04807020
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionUniversity of the Ryukyus

Principal Investigator

TOKU Seikichi  University of the Ryukyus, Biochemistry, instructor, 医学部, 助手 (10143091)

Co-Investigator(Kenkyū-buntansha) MAEDA Noriko  University of the Ryukyus, Biochemistry, instructor, 医学部, 教務職員 (40231584)
TANAKA Tatsuo  University of the Ryukyus, Biochemistry, Professor, 医学部, 教授 (70018688)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsribosomal protein L37a / ribosomal protein L7a / coordinate expression / carcinogenesis / oncogene / Ets oncoprotein / リボソーム蛋白 L37a / リボソーム蛋白質 / L7a / L37a / L5 / L30 / プロモーター / 転写因子 / ETS
Research Abstract

To reveal gene expression of ribosomal proteins and its regulation by oncoproteins, we characterized gene promoters of chicken ribosomal protein L7a and L37a in terms of cis-elements for nuclear protein binding and for transcriptional regulation. The promoter of L37a had seven protein binding regions (designated from upstream as A to G). Regions B contained two Ets oncoprotein binding sites (EBS), and its deletion resulted in drastic loss of promoter activity. Cooperation of both EBS for protein binding and promoter activity was observed. EMSA analysis of region C encompassing transcription initiation site revealed binding of a factor differing from TBP.Region D containing a sequence homologous to delta factor-binding element of mouse L32 gene located at the junction between exon I and intron I.Deletion of the region increased promoter activity about 5 folds, indicating repressive function reversed to the mouse element. Regions E to G constituted internal control region in the intron I.Moreover, we found quite similarities in sequence and in the position of regions, B, C, D, E and G among various r-protein gene promoters. It could be probable that these common elements are involved in the coordinate expression of r-protein genes, and that the non coordinate expression is caused by abnormality of oncoprotein such as Ets proteins.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Machida,M.,Toku,S.,Kenmochi,N.and Tanaka,T.: "The structure of the gene encoding chicken ribosomal protein L37a." Eur.J.Biochem.213. 77-80 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakasone,K.,Kenmochi,N.,Toku,S.and Tanaka,T.: "The structure of the gene encoding chicken ribosomal protein L30." Biochim.Biophys.Acta.1174. 75-78 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroi,Y.and Toku,S.: "Some properties of lysosomal arylamidase in rat liver." Biochem.Mol.Biol.Int.30. 705-711 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakasone,K.,Kenmochi,N.Seikichi,T.and Tanaka,T.: "The structure of chicken ribosomal protein L30." Ryukyu Med.J.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroi,Y.and Toku,S.: "Purification and characterization of an arylamidase from rat liver lysosomes." (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toku,S.and Tanaka,T.: "A chracterization of transcriptional regulatory elements in chicken ribosomal protein L37a gene." (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toku, S.and Tanaka, T.: "The primary structure of chicken ribosomal protein L37a." Biochim.Biophys.Acta.1132. 88-90 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Machida, M., Toku, S., Kenmochi, N.and Tanaka, T.: "The structure of the gene encoding chicken ribosomal protein L37a." Eur.J.Biochem.213. 77-80 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakasone, K., Kenmochi, N., Toku, S.and Tanaka, T.: "The structure of the gene encoding chicken ribosomal protein L30." Biochim.Biophys.Acta.1174. 75-78 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroi, Y.and Toku, S.: "Some properties of lysosomal arylamidase in rat liver." Biochem.Mol.Biol.Int.30. 705-711 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakasone, K., Kenmochi, N., Seikichi, T.and Tanaka, T.: "The structure of chicken ribosomal protein L30." Ryukyu Med.J.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroi, Y., and Toku, S.: "Purification and characterization of an arylamidase from rat liver lysosomes." under submission.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toku, S.and Tanaka, T.: "A characterization of transcriptional regulatory elements in chicken ribosomal protein L37a gene." under submission.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Machida,M.,Toku,S.,Kenmochi,N.and Tanaka,T: "The structure of the gene encoding chicken ribosomal protein L37a." Eur.J.Biochem.213. 77-80 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakasone,K.,Kenmochi,N.,Toku,S.and Tanaka,T.: "The structure of the gene encoding chicken ribosomal protein L30." Biochim.Biophys.Acta.1174. 75-78 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiroi,Y.and Toku,S.: "Some properties of lysosomal arylamidase in rat liver." Biochem.Mol.Biol.Int.30. 705-711 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakasone,K.,Kenmochi,N.,Seikichi,T.and Tanaka,T.: "The structure of chicken ribosomal protein L30." Ryukyu Med.J.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Seikichi Toku: "The primary structure of chicken ribosomal protein L37a" Biochimica et Biophysica Acta. 1132. 88-90 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Maki Machida: "The structure of the gene encoding chicken ribosomal protein L37a" European Journal of Biochemistry.

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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