Role of phosphosphing in bacter as a virulence factor
| Project/Area Number |
04807029
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | Gifu University |
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Principal Investigator |
YOSHINORI Muto Gifu University College of medical Science Associate Professor, 医療技術短期大学部, 助教授 (80190859)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Phosphosphingolipid / Anaerobic bacteria / Virulence factor / 病原性 |
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| Research Abstract |
Phosphosphingolipids extracted from 10 species of the genus Bacteroides were identified using thin-layr chromatography and infrared spectrophotometry after mild alkaline hydrolysis of the cellular lipids. All the species of genus Bacteroides tested contained phosphosphingolipids as their major components of cellular phospholipids. The two different types of phosphosphingolipid, ceramide phosphorylethanolamine and ceramide phosphorylglycerol, were detected in B.fragilis, B.ovatus, B.uniformis, B.caccae, B.eggerthii, B.thetaiotaomicron, and B.Stercoris, but not in B.merdae, B.distasonis, and B.vulgatus. The strains from genera Prevotella and porphyromonas also contained these two sphingolipids. These sphingolipid profiles were conserved within the species tested. The growth of cultured PC12 cells was significantly inhibited upon incubation with 10 mu g/ml of phosphosphingolipid isolated from B.fragilis cells. Exogenous phosphosphingolipids from Bacteroides also inhibited the cAMP-induced neurite outgrowth in cultured PC12 cells. Taken together, these results suggest that phosphosphingolipids widely distributed in Bacteroides species may play an important role as a virulence factor during bacterial infection.
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Report
(3 results)
Research Products
(10 results)
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[Publications] Watanabe, K., Ueno, K., Kato, N., Muto, Y., Bandoh, K., Tanabe, Y., Jotwani, R., Goto, M., Shimada, K., Shimizu, K., Goto, S.and Hara, K.: "In vitoro susceptibility of clinocal ispolates of Bacteroides fragilis and Bacteroides thetaiotaomicron in Japan." Eur.J.Clin.Microbiol. Infect.Dis.11. 1069-1073 (1992)
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[Publications] Tanaka, Y., Watanabe, K., Shimakura, Y., Bandoh, K., Muto, Y., Kato, N.and Ueno, K.: "Experimental mice intraabdominal abscess model for the evaluation of chemotherapeutic agents." CHEMOTHERAPY. 40. 1224-1230 (1992)
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[Publications] Kato, M., Muto, Y., Tanaka, K., Tanaka, Y., Watanabe, K.and Ueno, K.: "Distribution of phosphosphingolipids in the genus Bacteroides." Journal of Japanese Association for Anaerobic Infection Reseach. 23. 27-30 (1993)
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[Publications] Watanabe, K., Katoh, N., Muto, Y., Tanaka, K., Tanaka, Y., Katoh, H.and Ueno, K.: "In vitro antibacterial activity of novel carbacephem, lorcarbef, against anaerobic and aerobic bacteria." CHEMOTHERAPY. 41. 23-30 (1993)
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