| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
|
|---|
| Research Abstract |
Phosphosphingolipids extracted from 10 species of the genus Bacteroides were identified using thin-layr chromatography and infrared spectrophotometry after mild alkaline hydrolysis of the cellular lipids. All the species of genus Bacteroides tested contained phosphosphingolipids as their major components of cellular phospholipids. The two different types of phosphosphingolipid, ceramide phosphorylethanolamine and ceramide phosphorylglycerol, were detected in B.fragilis, B.ovatus, B.uniformis, B.caccae, B.eggerthii, B.thetaiotaomicron, and B.Stercoris, but not in B.merdae, B.distasonis, and B.vulgatus. The strains from genera Prevotella and porphyromonas also contained these two sphingolipids. These sphingolipid profiles were conserved within the species tested. The growth of cultured PC12 cells was significantly inhibited upon incubation with 10 mu g/ml of phosphosphingolipid isolated from B.fragilis cells. Exogenous phosphosphingolipids from Bacteroides also inhibited the cAMP-induced neurite outgrowth in cultured PC12 cells. Taken together, these results suggest that phosphosphingolipids widely distributed in Bacteroides species may play an important role as a virulence factor during bacterial infection.
|
